Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.8.3.3 extracted from

  • Huang, Z.; Villarta-Snow, R.L.; Lubrano, G.J.; Guilbault, G.G.
    Glutathione amperometric enzyme microsensor (1994), Anal. Lett., 27, 263-271.
No PubMed abstract available
Search for more literature for 1.8.3.3

Application

Application Comment Organism
analysis development of a glutathione micro-enzyme sensor for amperometric detection in vivo, immobilization of enzyme on microelectrode tips Penicillium sp.

General Stability

General Stability Organism
after 36 measurements with enzyme immobilized on microelectrode sensor loss of 30% activity Penicillium sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.7
-
 glutathione immobilized enzyme on sensor surface out of cellulose acetate Penicillium sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
glutathione + O2 Penicillium sp. measurement of glutathione in vivo via microelectrodes with immobilized enzyme glutathione disulfide + H2O2
-
 ?

Organism

Organism UniProt Comment Textmining
Penicillium sp.
-
-
-

Storage Stability

Storage Stability Organism
4°C, phosphate buffered saline, immobilized enzyme on microelectrode sensor, loss of 20% activity, 68 days Penicillium sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
cysteine-glycine + O2 enzyme immobilized on microelectrode sensor Penicillium sp. ?
-
 ?
glutathione + O2 enzyme immobilized on microsensor Penicillium sp. glutathione disulfide + H2O2
-
 ?
glutathione + O2 measurement of glutathione in vivo via microelectrodes with immobilized enzyme Penicillium sp. glutathione disulfide + H2O2
-
 ?
S-methylglutathione + O2 enzyme immobilized on microelectrode sensor, low activity Penicillium sp. ?
-
 ?

pH Range

pH Minimum pH Maximum Comment Organism
3.8 11 enzyme immobilized on microsensor surface out of cellulose acetate Penicillium sp.

Cofactor

Cofactor Comment Organism Structure
FAD flavoprotein Penicillium sp.