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Literature summary for 1.8.3.2 extracted from
- Sulfhydryl oxidases as factors for mitochondrial biogenesis (2002), Methods Enzymol., 348, 314-324.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression of His6-tagged Alrp in Escherichia coli, expression of GFP-fusion enzyme in cell culture | Homo sapiens |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | the conserved C-terminal domain of the human Alrp can functionally replace the yeast domain in vivo, genetic system to study function of sulfhydryl oxidases, overview | Homo sapiens |
| additional information | the conserved C-terminal domain of the human Alrp can functionally replace the yeast domain in vivo, genetic system to study function of sulfhydryl oxidases, overview, enzyme-defective Erv1p mutant shows highly altered mitochondrial membrane morphology with loss of cristae, overview | Saccharomyces cerevisiae |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| membrane | - |
Homo sapiens | 16020 | - |
| membrane | - |
Saccharomyces cerevisiae | 16020 | - |
| mitochondrion | - |
Homo sapiens | 5739 | - |
| mitochondrion | - |
Saccharomyces cerevisiae | 5739 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 15000 | - |
2 * 22000, long Erv1p form, SDS-PAGE, 2 * 15000, short Evrp1 form, SDS-PAGE | Saccharomyces cerevisiae |
| 22000 | - |
2 * 22000, long Erv1p form, SDS-PAGE, 2 * 15000, short Evrp1 form, SDS-PAGE | Saccharomyces cerevisiae |
| 44000 | - |
Evr1p | Saccharomyces cerevisiae |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | Homo sapiens | the enzyme is involved in mitochondrial biogenesis | ? | - |
? |  |
| additional information | Saccharomyces cerevisiae | the enzyme is involved in mitochondrial biogenesis | ? | - |
? | |
| R-SH + O2 | Homo sapiens | - |
R-S-S-R + H2O2 | - |
ir | |
| R-SH + O2 | Saccharomyces cerevisiae | - |
R-S-S-R + H2O2 | - |
ir | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
Alrp protein | - |
| Saccharomyces cerevisiae | - |
- |
- |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| 2 R'C(R)SH + O2 = R'C(R)S-S(R)CR' + H2O2 | enzyme contains a functionally involved redox-active motif CXXC | Homo sapiens | |
| 2 R'C(R)SH + O2 = R'C(R)S-S(R)CR' + H2O2 | enzyme contains a functionally involved redox-active motif CXXC | Saccharomyces cerevisiae |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| additional information | enzyme is expressed in a large number of different cell types and tissues | Homo sapiens | - |
| additional information | enzyme is expressed in a large number of different cell types and tissues | Saccharomyces cerevisiae | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Homo sapiens |
| additional information | - |
- |
Saccharomyces cerevisiae |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | the enzyme is involved in mitochondrial biogenesis | Homo sapiens | ? | - |
? | |
| additional information | the enzyme is involved in mitochondrial biogenesis | Saccharomyces cerevisiae | ? | - |
? | |
| R-SH + O2 | - |
Homo sapiens | R-S-S-R + H2O2 | - |
ir | |
| R-SH + O2 | - |
Saccharomyces cerevisiae | R-S-S-R + H2O2 | - |
ir | |
| reduced lysozyme + O2 | - |
Saccharomyces cerevisiae | lysozyme disulfide + H2O2 | - |
ir | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | - |
Homo sapiens |
| dimer | 2 * 22000, long Erv1p form, SDS-PAGE, 2 * 15000, short Evrp1 form, SDS-PAGE | Saccharomyces cerevisiae |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| ALRp | - |
Homo sapiens |
| Erv1p | - |
Saccharomyces cerevisiae |
| sulfhydryl oxidase | - |
Homo sapiens |
| sulfhydryl oxidase | - |
Saccharomyces cerevisiae |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | - |
Homo sapiens | |
| additional information | - |
additional information | - |
Saccharomyces cerevisiae |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
assay at | Homo sapiens |
| 7.5 | - |
assay at | Saccharomyces cerevisiae |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| FAD | dependent on | Homo sapiens | |
| FAD | dependent on | Saccharomyces cerevisiae | |
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Release 2023.1 (January 2023)
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