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Literature summary for 1.8.3.2 extracted from
- Sulphadryl oxidase: properties and application (1986), Enzyme Microb. Technol., 2, 265-272.
No PubMed abstract available
Application
| Application | Comment | Organism |
|---|---|---|
| nutrition | elimination of cooked flavour in ultra-high temperature commercially sterile milk, may have other applications for flavour modification | Bos taurus |
General Stability
| General Stability | Organism |
|---|---|
| crosslinked immobilized enzyme is very stable to urea | Bos taurus |
| immobilized enzyme, 10% ethanol, 0.01% H2O2, or in 0.02% sodium azide, loss of only small amounts of activity | Bos taurus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| diazabicyclooctane | - |
Bos taurus |  |
| guanine | - |
Bos taurus | |
| o-Dianisidine | - |
Bos taurus | |
| o-phenylenediamine | - |
Bos taurus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.3 | - |
GSH | - |
Bos taurus | |
| 0.8 | - |
L-Cys | - |
Bos taurus | |
| 1.33 | - |
D-Cys | - |
Bos taurus | |
| 3.85 | - |
N-acetyl-L-Cys | - |
Bos taurus | |
| 30 | - |
cysteamine | - |
Bos taurus | |
| 100 | - |
2-nitro-5-thiobenzoic acid | - |
Bos taurus | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Iron | contains enzyme-bound iron | Bos taurus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 89000 | - |
x * 89000, SDS-PAGE | Bos taurus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bos taurus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Bos taurus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| milk | - |
Bos taurus | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2-nitro-5-thiobenzoic acid + O2 | - |
Bos taurus | ? + H2O | - |
? | |
| cysteamine + O2 | - |
Bos taurus | ? + H2O | - |
? | |
| D-Cys + O2 | - |
Bos taurus | ? + H2O | - |
? | |
| GSH + O2 + O2 | - |
Bos taurus | GSSG + H2O | - |
? | |
| L-Cys + O2 | - |
Bos taurus | ? + H2O | - |
? | |
| additional information | enzyme does not catalyze thiol-disulfide interchange | Bos taurus | ? | - |
? | |
| N-acetylcysteine + O2 | - |
Bos taurus | ? + H2O | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 89000, SDS-PAGE | Bos taurus |
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