Literature summary for 1.8.3.1 extracted from
Kalimuthu, P.; Belaidi, A.; Schwarz, G.; Bernhardt, P.
Low potential catalytic voltammetry of human sulfite oxidase (2016), Electrochim. Acta, 199, 280-289 .
No PubMed abstract available
Application
Application |
Comment |
Organism |
analysis |
an enzyme-coupled electrode is successfully used for the determination of sulfite concentration in white wine and beer samples, enzyme electrode preparation and method evaluation |
Homo sapiens |
Cloned(Commentary)
Cloned (Comment) |
Organism |
recombinant enzyme expression in Escherichia coli strain TP1000 |
Homo sapiens |
Protein Variants
Protein Variants |
Comment |
Organism |
additional information |
mediated electrocatalytic voltammetry of human sulfite oxidase (HSO) is demonstrated with synthetic one electron transfer iron complexes bis(1,4,7-triazacyclononane)iron(III) ([Fe(tacn)2]3+) and 1,2-bis(1,4,7-triaza-1-cyclononyl)ethane iron(III) ([Fe(dtne)]3+) at a glassy carbon working electrode, enzyme-dependent kinetics, overview. The HSO coupled electrode is successfully used for the determination of sulfite concentration in white wine and beer samples, and the results validate with a standard spectrophotometric method |
Homo sapiens |
Metals/Ions
Metals/Ions |
Comment |
Organism |
Structure |
Iron |
- |
Homo sapiens |
|
Molybdenum |
in the catalytic reaction, SO is active in its fully oxidized state (MoVI) in which molybdenum is coordinated by a cysteine thiolate, the dithiolene group of molybdopterin, and two terminal oxygen atoms |
Homo sapiens |
|
Natural Substrates/ Products (Substrates)
Natural Substrates |
Organism |
Comment (Nat. Sub.) |
Natural Products |
Comment (Nat. Pro.) |
Rev. |
Reac. |
sulfite + O2 + H2O |
Homo sapiens |
- |
sulfate + H2O2 |
- |
? |
|
Organism
Organism |
UniProt |
Comment |
Textmining |
Homo sapiens |
P51687 |
- |
- |
Reaction
Reaction |
Comment |
Organism |
Reaction ID |
sulfite + O2 + H2O = sulfate + H2O2 |
electrocatalytic mechanism of HSO, overview |
Homo sapiens |
|
Substrates and Products (Substrate)
Substrates |
Comment Substrates |
Organism |
Products |
Comment (Products) |
Rev. |
Reac. |
sulfite + O2 + H2O |
- |
Homo sapiens |
sulfate + H2O2 |
- |
? |
|
Synonyms
Synonyms |
Comment |
Organism |
HSO |
- |
Homo sapiens |
pH Optimum
pH Optimum Minimum |
pH Optimum Maximum |
Comment |
Organism |
8.5 |
- |
- |
Homo sapiens |
pH Range
pH Minimum |
pH Maximum |
Comment |
Organism |
6 |
11 |
pH dependence of the maximum catalytic oxidation current at the GC/HSO electrode with 4 mM sulfite and in the presence of 10 mM [Fe(tacn)]23+ in 100 mM mixed buffer solution, overview |
Homo sapiens |
Cofactor
Cofactor |
Comment |
Organism |
Structure |
cytochrome c |
natural electron acceptor cytochrome c |
Homo sapiens |
|
heme |
the heme accepts electrons from the Mo ion following sulfite oxidation, a flexible connects the Mo and heme domains |
Homo sapiens |
|
molybdenum cofactor |
the heme accepts electrons from the Mo ion following sulfite oxidation, a flexible connects the Mo and heme domains |
Homo sapiens |
|
additional information |
vertebrate sulfite oxidases can use either cytochrome c or dioxygen as an electron acceptor |
Homo sapiens |
|