Activating Compound | Comment | Organism | Structure |
---|---|---|---|
EDTA | - |
Thermus thermophilus | |
Molybdenum | the addition of molybdenum to culture media at a concentration of 2.07 mM molybdate leads to a 4fold increase in activity | Thermus thermophilus | |
additional information | molybdenum concentrations higher than 2.07 mM do not show increase of enzyme activity | Thermus thermophilus |
Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli strain TP1000 | Thermus thermophilus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
arsenite | 7.4 mM, 50% inhibition | Thermus thermophilus | |
Ca2+ | - |
Thermus thermophilus | |
additional information | inhibition at 400 mM salt concentrations; no inhibition by periodate or 80 mM sulfate | Thermus thermophilus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
periplasm | - |
Thermus thermophilus | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
K+ | 45-100 mM, 54-88% increase of activity | Thermus thermophilus | |
Molybdenum | the addition of molybdenum to culture media at a concentration of 2.07 mM molybdate leads to a 4fold increase in activity | Thermus thermophilus | |
NH4+ | 30 mM, 74% increase of activity | Thermus thermophilus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
38000 | - |
gel filtration | Thermus thermophilus |
39100 | - |
SDS-PAGE | Thermus thermophilus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermus thermophilus | - |
- |
- |
Thermus thermophilus AT62 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
DEAE 52 column chromatography, Superdex X26 gel filtration and hydroxyapatite column chromatography | Thermus thermophilus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.191 | - |
crude cell extract | Thermus thermophilus |
56.67 | - |
after 297fold purification | Thermus thermophilus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
sulfite + H2O + A | - |
Thermus thermophilus | SO42- + AH2 | - |
? | |
sulfite + H2O + A | - |
Thermus thermophilus AT62 | SO42- + AH2 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
SO | - |
Thermus thermophilus |
sulfite oxidase | - |
Thermus thermophilus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
- |
Thermus thermophilus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | 80 | The enzyme retains 100% of residual activity up to 7 h of incubation at 50°C. Half life times of the enzyme at 60, 70 and 80°C are respectively 8, 6.5 and 1.5 h. The enzyme retains 30% of activity at 25°C and 45% at 90°C. | Thermus thermophilus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.4 | - |
sulfite | - |
Thermus thermophilus | |
53.32 | - |
sulfite | - |
Thermus thermophilus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
11 | - |
- |
Thermus thermophilus |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
9.4 | - |
Ca2+ | - |
Thermus thermophilus |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Thermus thermophilus | - |
- |
6.8 |