Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21 Star (DE3) cells | Thioalkalivibrio paradoxus |
Crystallization (Comment) | Organism |
---|---|
hanging drop vapor diffusion method, using 0.17 M ammonium acetate, 0.085 M sodium citrate, pH 5.6, 25.5% (w/v) PEG 4000, 15% (v/v) glycerol or 0.17 M ammonium sulfate, 0.085 M sodium cacodylate, pH 6.5, 25.5% (w/v) PEG 8000, 15% (v/v) glycerol | Thioalkalivibrio paradoxus |
Protein Variants | Comment | Organism |
---|---|---|
E288A | inactive | Thioalkalivibrio paradoxus |
H136A | inactive | Thioalkalivibrio paradoxus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
azide | - |
Thioalkalivibrio paradoxus | |
azide | - |
Thioalkalivibrio thiocyanoxidans | |
Cyanate | mixed-type inhibition | Thioalkalivibrio paradoxus | |
Cyanate | mixed-type inhibition | Thioalkalivibrio thiocyanoxidans | |
cyanide | competitive inhibition | Thioalkalivibrio paradoxus | |
cyanide | competitive inhibition | Thioalkalivibrio thiocyanoxidans | |
additional information | nitrate, nitrite, bicarbonate, sulfate, acetate, and iodide show no inhibitory effect at 1 mM | Thioalkalivibrio paradoxus | |
additional information | nitrate, nitrite, bicarbonate, sulfate, acetate, and iodide show no inhibitory effect at 1 mM | Thioalkalivibrio thiocyanoxidans |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.8 | - |
Wurster's blue | at pH 9.5 and 30°C | Thioalkalivibrio paradoxus | |
1 | - |
ferricytochrome c 550 | at pH 9.5 and 30°C | Thioalkalivibrio paradoxus | |
1.2 | - |
potassium ferricyanide | at pH 9.5 and 30°C | Thioalkalivibrio paradoxus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Cu2+ | contains three copper ions | Thioalkalivibrio paradoxus | |
Cu2+ | contains three copper ions | Thioalkalivibrio thiocyanoxidans |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thioalkalivibrio paradoxus | - |
- |
- |
Thioalkalivibrio paradoxus ARh1 | - |
- |
- |
Thioalkalivibrio thiocyanoxidans | - |
- |
- |
Thioalkalivibrio thiocyanoxidans ARh2 | - |
- |
- |
Thioalkalivibrio thiocyanoxidans ARh4 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
DEAE-Sepharose column chromatography and Superdex 200 gel filtration | Thioalkalivibrio paradoxus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | low-potential electron acceptors, such as 2,3,5-triphenyltetrazolium chloride and methylene blue, are not reduced. Direct oxidation of thiocyanate by molecular oxygen is not observed | Thioalkalivibrio paradoxus | ? | - |
- |
|
additional information | low-potential electron acceptors, such as 2,3,5-triphenyltetrazolium chloride and methylene blue, are not reduced. Direct oxidation of thiocyanate by molecular oxygen is not observed | Thioalkalivibrio thiocyanoxidans | ? | - |
- |
|
additional information | low-potential electron acceptors, such as 2,3,5-triphenyltetrazolium chloride and methylene blue, are not reduced. Direct oxidation of thiocyanate by molecular oxygen is not observed | Thioalkalivibrio paradoxus ARh1 | ? | - |
- |
|
additional information | low-potential electron acceptors, such as 2,3,5-triphenyltetrazolium chloride and methylene blue, are not reduced. Direct oxidation of thiocyanate by molecular oxygen is not observed | Thioalkalivibrio thiocyanoxidans ARh2 | ? | - |
- |
|
additional information | low-potential electron acceptors, such as 2,3,5-triphenyltetrazolium chloride and methylene blue, are not reduced. Direct oxidation of thiocyanate by molecular oxygen is not observed | Thioalkalivibrio thiocyanoxidans ARh4 | ? | - |
- |
|
thiocyanate + 2 ferricytochrome c 550 + H2O | - |
Thioalkalivibrio paradoxus | cyanate + sulfur + 2 ferrocytochrome c 550 + 2 H+ | - |
? | |
thiocyanate + 2 ferricytochrome c 550 + H2O | - |
Thioalkalivibrio thiocyanoxidans | cyanate + sulfur + 2 ferrocytochrome c 550 + 2 H+ | - |
? | |
thiocyanate + 2 ferricytochrome c 550 + H2O | - |
Thioalkalivibrio paradoxus ARh1 | cyanate + sulfur + 2 ferrocytochrome c 550 + 2 H+ | - |
? | |
thiocyanate + 2 ferricytochrome c 550 + H2O | - |
Thioalkalivibrio thiocyanoxidans ARh2 | cyanate + sulfur + 2 ferrocytochrome c 550 + 2 H+ | - |
? | |
thiocyanate + 2 ferricytochrome c 550 + H2O | - |
Thioalkalivibrio thiocyanoxidans ARh4 | cyanate + sulfur + 2 ferrocytochrome c 550 + 2 H+ | - |
? | |
thiocyanate + 2 potassium ferricyanide + H2O | - |
Thioalkalivibrio paradoxus | cyanate + sulfur + 2 potassium ferrocyanide + 2 H+ | - |
? | |
thiocyanate + 2 potassium ferricyanide + H2O | - |
Thioalkalivibrio thiocyanoxidans | cyanate + sulfur + 2 potassium ferrocyanide + 2 H+ | - |
? | |
thiocyanate + 2 potassium ferricyanide + H2O | - |
Thioalkalivibrio paradoxus ARh1 | cyanate + sulfur + 2 potassium ferrocyanide + 2 H+ | - |
? | |
thiocyanate + 2 potassium ferricyanide + H2O | - |
Thioalkalivibrio thiocyanoxidans ARh2 | cyanate + sulfur + 2 potassium ferrocyanide + 2 H+ | - |
? | |
thiocyanate + 2 potassium ferricyanide + H2O | - |
Thioalkalivibrio thiocyanoxidans ARh4 | cyanate + sulfur + 2 potassium ferrocyanide + 2 H+ | - |
? | |
thiocyanate + Wurster's blue + H2O | - |
Thioalkalivibrio thiocyanoxidans | cyanate + sulfur + reduced Wurster's blue + 2 H+ | - |
? | |
thiocyanate + Wurster's blue + H2O | - |
Thioalkalivibrio thiocyanoxidans ARh2 | cyanate + sulfur + reduced Wurster's blue + 2 H+ | - |
? | |
thiocyanate + Wurster's blue + H2O | - |
Thioalkalivibrio thiocyanoxidans ARh4 | cyanate + sulfur + reduced Wurster's blue + 2 H+ | - |
? | |
thiocyanate + Wurster's blue + H2O | - |
Thioalkalivibrio paradoxus | cyanate + sulfur + reduced Wurster's blue + H+ | - |
? | |
thiocyanate + Wurster's blue + H2O | - |
Thioalkalivibrio thiocyanoxidans | cyanate + sulfur + reduced Wurster's blue + H+ | - |
? | |
thiocyanate + Wurster's blue + H2O | - |
Thioalkalivibrio paradoxus ARh1 | cyanate + sulfur + reduced Wurster's blue + H+ | - |
? | |
thiocyanate + Wurster's blue + H2O | - |
Thioalkalivibrio thiocyanoxidans ARh2 | cyanate + sulfur + reduced Wurster's blue + H+ | - |
? | |
thiocyanate + Wurster's blue + H2O | - |
Thioalkalivibrio thiocyanoxidans ARh4 | cyanate + sulfur + reduced Wurster's blue + H+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | 2 * 51891, calculated from amino acid sequence | Thioalkalivibrio paradoxus |
homodimer | 2 * 51891, calculated from amino acid sequence | Thioalkalivibrio thiocyanoxidans |
homodimer | 2 * 52000-53000, SDS-PAGE | Thioalkalivibrio paradoxus |
homodimer | 2 * 52000-53000, SDS-PAGE | Thioalkalivibrio thiocyanoxidans |
Synonyms | Comment | Organism |
---|---|---|
TcDH | - |
Thioalkalivibrio paradoxus |
TcDH | - |
Thioalkalivibrio thiocyanoxidans |
thiocyanate dehydrogenase | - |
Thioalkalivibrio paradoxus |
thiocyanate dehydrogenase | - |
Thioalkalivibrio thiocyanoxidans |
thiocyanate: cytochrome c oxidoreductase | - |
Thioalkalivibrio paradoxus |
thiocyanate: cytochrome c oxidoreductase | - |
Thioalkalivibrio thiocyanoxidans |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9.5 | - |
- |
Thioalkalivibrio paradoxus |
9.5 | - |
- |
Thioalkalivibrio thiocyanoxidans |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
8 | 11 | more than 50% activity between pH 8.0 and 11.0 with potassium ferricyanide as electron acceptor, more than 50% activity between pH 8.5 and 10.5 with potassium ferricyanide as electron acceptor | Thioalkalivibrio paradoxus |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0014 | - |
cyanide | at pH 9.5 and 30°C | Thioalkalivibrio paradoxus |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
60 | - |
at pH 9.5 and 30°C | Thioalkalivibrio paradoxus | azide |