Literature summary for 1.8.2.2 extracted from

Silver, M.; Lundgren, D.G.
The thiosulfate-oxidizing enzyme of Ferrobacillus ferrooxidans (Thiobacillus ferrooxidans) (1968), Can. J. Biochem., 46, 1215-1220.

Search for more literature for 1.8.2.2

Inhibitors

Inhibitors	Comment	Organism	Structure
azide	60% inhibition at 10 mM	Acidithiobacillus ferrooxidans
cyanide	28.9% inhibition at 10 mM	Acidithiobacillus ferrooxidans
HgCl2	73.3% inhibition at 1 mM	Acidithiobacillus ferrooxidans

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
additional information	-	additional information	-	Acidithiobacillus ferrooxidans
0.9	-	thiosulfate	-	Acidithiobacillus ferrooxidans

Organism

Organism	UniProt	Comment	Textmining
Acidithiobacillus ferrooxidans	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
using heat-treatment, 5-17% ethanol precipitation and CM-cellulose treatment	Acidithiobacillus ferrooxidans

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
additional information	-	-	Acidithiobacillus ferrooxidans

Storage Stability

Storage Stability	Organism
-20°C, little loss of activity after 2 months	Acidithiobacillus ferrooxidans
diluted enzyme loses 10-15% of the activity each time it is frozen and thawed	Acidithiobacillus ferrooxidans

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
thiosulfate + oxidized acceptor	the activity does not couple with mammalian cytochrome c as electron acceptor	Acidithiobacillus ferrooxidans	tetrathionate + reduced acceptor	-	ir
thiosulfate + oxidized acceptor	ferricyanide acts as electron acceptor	Acidithiobacillus ferrooxidans	tetrathionate + reduced acceptor	-	ir
thiosulfate + oxidized acceptor	the enzyme is active only with ferricyanide, not with native or mammalian cytochrome c	Acidithiobacillus ferrooxidans	tetrathionate + reduced acceptor	-	ir

Temperature Stability [°C]

Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
100	-	complete loss of activity	Acidithiobacillus ferrooxidans

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
5	-	-	Acidithiobacillus ferrooxidans

pH Stability

pH Stability	pH Stability Maximum	Comment	Organism
4	-	unstable below	Acidithiobacillus ferrooxidans

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Release 2023.1 (January 2023)