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Literature summary for 1.8.2.2 extracted from
- Thiosulfate oxidase from an Alcaligenes grown on mercaptosuccinate (1972), Can. J. Microbiol., 18, 235-245.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| EDTA | 18% inhibition at 1 mM and 46% inhibition at 20 mM | Alcaligenes sp. |  |
| HgCl2 | 100% inhibition at 1 mM | Alcaligenes sp. | |
| N-ethylmaleimide | 78% inhibition at 1 mM | Alcaligenes sp. | |
| p-chloromercuribenzoate | 90% inhibition at 1 mM | Alcaligenes sp. | |
| SO32- | 62% inhibition at 3 mM | Alcaligenes sp. | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 90000 | - |
gel filtration | Alcaligenes sp. |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Alcaligenes sp. | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| using MnCl2 precipitation, treatment with calcium phosphate gel, and successive column chromatography on DEAE-Sephadex and Sephadex G-150 | Alcaligenes sp. |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
specific activities in crude cell-free extracts of mercaptosuccinate-grown Alcaligenes under a variety of nutritional conditions | Alcaligenes sp. |
| 6010 | - |
- |
Alcaligenes sp. |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 4°C, purified enzyme, 10% loss of activity after 24 h, completely inactive after 7 days | Alcaligenes sp. |
| frozen, 0.05 M phosphate buffer, pH 6.2, stable for at least 90 days | Alcaligenes sp. |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | NAD+, methylene blue, 2,6-dichlorophenol indophenol | Alcaligenes sp. | ? | - |
? | |
| additional information | no activity with FAD | Alcaligenes sp. | ? | - |
? | |
| thiosulfate + oxidized acceptor | strictly specific for thiosulfate | Alcaligenes sp. | tetrathionate + reduced acceptor | - |
? | |
| thiosulfate + oxidized acceptor | ferricyanide or native cytochrome c act as electron acceptors | Alcaligenes sp. | tetrathionate + reduced acceptor | - |
? | |
| thiosulfate + oxidized acceptor | cytochrome c acts as electron acceptor | Alcaligenes sp. | tetrathionate + reduced acceptor | - |
? | |
| thiosulfate + oxidized acceptor | ferricyanide acts as electron acceptor | Alcaligenes sp. | tetrathionate + reduced acceptor | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
- |
Alcaligenes sp. |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 22 | 48 | about 50% of activity maximum at 22°C and 48°C | Alcaligenes sp. |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
30 min, 7% loss of activity | Alcaligenes sp. |
| 45 | - |
30 min, complete loss of activity | Alcaligenes sp. |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5 | - |
below, using ferricyanide as electron acceptor | Alcaligenes sp. |
| 7.2 | 7.6 | cytochrome c as electron acceptor | Alcaligenes sp. |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 9 | - |
Alcaligenes sp. |
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Release 2023.1 (January 2023)
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