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Literature summary for 1.8.2.2 extracted from

  • Schook, L.B.; Berk, R.S.
    Partial purification and characterization of thiosulfate oxidase from Pseudomonas aeruginosa (1979), J. Bacteriol., 140, 306-308.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.8.2.2

Inhibitors

Inhibitors Comment Organism Structure
dithionate 18% inhibition at 1 mM, 15% inhibition at 0.1 mM and 0.01 mM Pseudomonas aeruginosa
EDTA 13% inhibition at 1 mM, 3% inhibition at 0.1 mM Pseudomonas aeruginosa
HgCl2 37% inhibition at 0.1 mM, 13% inhibition at 0.01 mM Pseudomonas aeruginosa
N-ethylmaleimide 40% inhibition at 1 mM, 15% inhibition at 0.1 mM Pseudomonas aeruginosa
p-chloromercuribenzoate 47% inhibition at 1 mM, 7% inhibition at 0.1 mM Pseudomonas aeruginosa
SO32- 75% inhibition at 1 mM, 48% inhibition at 0.1 mM 33% inhibition at 0.01 mM Pseudomonas aeruginosa
SO42- 33% inhibition at 1 mM, 15% inhibition at 0.1 mM, 7% inhibition at 0.01 mM Pseudomonas aeruginosa

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information
-
 Pseudomonas aeruginosa
0.67
-
 thiosulfate
-
 Pseudomonas aeruginosa
1.1
-
 ferricyanide
-
 Pseudomonas aeruginosa

Organism

Organism UniProt Comment Textmining
Pseudomonas aeruginosa
-
-
-

Purification (Commentary)

Purification (Comment) Organism
partial, using ultracentrifugation, ultrafiltration, column chromatography on DEAE-Sephadex A-25 and gel filtration on Sephadex S-200 Pseudomonas aeruginosa

Storage Stability

Storage Stability Organism
4°C, 50% loss of activity after 3 days, 75% loss of activity after 14 days Pseudomonas aeruginosa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
thiosulfate + ferricyanide
-
 Pseudomonas aeruginosa tetrathionate + ferrocyanide
-
 ?
thiosulfate + oxidized acceptor ferricyanide or native cytochrome c act as electron acceptors Pseudomonas aeruginosa tetrathionate + reduced acceptor
-
 ?
thiosulfate + oxidized acceptor cytochrome c acts as electron acceptor Pseudomonas aeruginosa tetrathionate + reduced acceptor
-
 ?
thiosulfate + oxidized acceptor mammalian cytochrome c acts as electron acceptor Pseudomonas aeruginosa tetrathionate + reduced acceptor
-
 ?
thiosulfate + oxidized acceptor ferricyanide acts as electron acceptor Pseudomonas aeruginosa tetrathionate + reduced acceptor
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30 42
-
 Pseudomonas aeruginosa

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.5 6.5
-
 Pseudomonas aeruginosa

pH Range

pH Minimum pH Maximum Comment Organism
4.5 9
-
 Pseudomonas aeruginosa