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Literature summary for 1.8.2.2 extracted from
- Cellular location and partial purification of the 'thiosulphate-oxidizing enzyme' and the 'trithionate hydrolase' from Thiobacillus tepidarius (1988), J. Gen. Microbiol., 134, 877-885.
No PubMed abstract available
General Stability
| General Stability | Organism |
|---|---|
| lability of enzyme during purification and storage at -20°C | Thermithiobacillus tepidarius |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.004 | - |
thiosulfate | with cytochrome c as electron acceptor | Thermithiobacillus tepidarius |  |
| 0.11 | - |
thiosulfate | with ferricyanide as electron acceptor | Thermithiobacillus tepidarius | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| periplasm | at least 73% of the enzyme activity is recovered in the periplasmic fraction | Thermithiobacillus tepidarius | - |
- |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 45000 | - |
3 * 45000, SDS-PAGE | Thermithiobacillus tepidarius |
| 138000 | - |
gel filtration | Thermithiobacillus tepidarius |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| thiosulfate + cytochrome c | Thermithiobacillus tepidarius | - |
tetrathionate + reduced cytochrome c | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Thermithiobacillus tepidarius | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| partial, using ion exchange chromatography, gel filtration and hydrophobic interaction chromatography | Thermithiobacillus tepidarius |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 1 | - |
cytoplasm | Thermithiobacillus tepidarius |
| 2 | - |
membrane | Thermithiobacillus tepidarius |
| 9.6 | - |
periplasm | Thermithiobacillus tepidarius |
| 316 | - |
partially purified enzyme | Thermithiobacillus tepidarius |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 20-30% loss of activity after freezing at -20°C and thawing | Thermithiobacillus tepidarius |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| thiosulfate + cytochrome c | - |
Thermithiobacillus tepidarius | tetrathionate + reduced cytochrome c | - |
? | |
| thiosulfate + oxidized acceptor | ferricyanide or native cytochrome c act as electron acceptors | Thermithiobacillus tepidarius | tetrathionate + reduced acceptor | - |
? | |
| thiosulfate + oxidized acceptor | cytochrome c acts as electron acceptor | Thermithiobacillus tepidarius | tetrathionate + reduced acceptor | - |
? | |
| thiosulfate + oxidized acceptor | ferricyanide acts as electron acceptor | Thermithiobacillus tepidarius | tetrathionate + reduced acceptor | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| trimer | 3 * 45000, SDS-PAGE | Thermithiobacillus tepidarius |
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