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Literature summary for 1.8.2.1 extracted from

  • Johnson-Winters, K.; Nordstrom, A.R.; Emesh, S.; Astashkin, A.V.; Rajapakshe, A.; Berry, R.E.; Tollin, G.; Enemark, J.H.
    Effects of interdomain tether length and flexibility on the kinetics of intramolecular electron transfer in human sulfite oxidase (2010), Biochemistry, 49, 1290-1296.
    View publication on PubMedView publication on EuropePMC

Protein Variants

Protein Variants Comment Organism
P105A the mutant enzyme shows increased catalytic efficiency compared to the wild type enzyme Homo sapiens
P105A/P111A the mutant enzyme shows about 30% decreased catalytic efficiency compared to the wild type enzyme Homo sapiens
P111A the mutant enzyme shows about 30% decreased catalytic efficiency compared to the wild type enzyme Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0026
-
sulfite tether deletion mutant enzyme DELTAKVATV, in 20 mM Tris acetate, pH 8.0, at 25°C Homo sapiens
0.006
-
sulfite mutant enzyme P105A, in 20 mM Tris acetate, pH 8.0, at 25°C Homo sapiens
0.0111
-
sulfite wild type enzyme, in 20 mM Tris acetate, pH 8.0, at 25°C Homo sapiens
0.026
-
sulfite tether deletion mutant enzyme DELTAKVAT, in 20 mM Tris acetate, pH 8.0, at 25°C Homo sapiens
0.032
-
sulfite mutant enzyme P105A/P111A, in 20 mM Tris acetate, pH 8.0, at 25°C Homo sapiens
0.033
-
sulfite mutant enzyme P111A, in 20 mM Tris acetate, pH 8.0, at 25°C Homo sapiens
0.042
-
sulfite tether deletion mutant enzyme DELTAKVA, in 20 mM Tris acetate, pH 8.0, at 25°C Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
mitochondrion
-
Homo sapiens 5739
-

Metals/Ions

Metals/Ions Comment Organism Structure
Molybdenum molybdenum enzyme Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
sulfite + ferricytochrome c + H2O Homo sapiens ferricytochrome c is the physiological electron acceptor sulfate + ferrocytochrome c + H+
-
?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
phenyl Sepharose column chromatography and Superdex 200 gel filtration Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
sulfite + ferricytochrome c + H2O ferricytochrome c is the physiological electron acceptor Homo sapiens sulfate + ferrocytochrome c + H+
-
?

Synonyms

Synonyms Comment Organism
sulfate oxidase
-
Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
10.6
-
sulfite tether deletion mutant enzyme DELTAKVATV, in 20 mM Tris acetate, pH 8.0, at 25°C Homo sapiens
26.9
-
sulfite wild type enzyme, in 20 mM Tris acetate, pH 8.0, at 25°C Homo sapiens
35
-
sulfite tether deletion mutant enzyme DELTAKVAT, in 20 mM Tris acetate, pH 8.0, at 25°C Homo sapiens
39
-
sulfite mutant enzyme P105A, in 20 mM Tris acetate, pH 8.0, at 25°C Homo sapiens
40
-
sulfite tether deletion mutant enzyme DELTAKVA, in 20 mM Tris acetate, pH 8.0, at 25°C Homo sapiens
46
-
sulfite mutant enzyme P105A/P111A, in 20 mM Tris acetate, pH 8.0, at 25°C Homo sapiens
50
-
sulfite mutant enzyme P111A, in 20 mM Tris acetate, pH 8.0, at 25°C Homo sapiens

Cofactor

Cofactor Comment Organism Structure
heme contains b-type heme Homo sapiens

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
950
-
sulfite tether deletion mutant enzyme DELTAKVA, in 20 mM Tris acetate, pH 8.0, at 25°C Homo sapiens
1400
-
sulfite tether deletion mutant enzyme DELTAKVAT, in 20 mM Tris acetate, pH 8.0, at 25°C Homo sapiens
1500
-
sulfite mutant enzyme P105A/P111A, in 20 mM Tris acetate, pH 8.0, at 25°C Homo sapiens
1500
-
sulfite mutant enzyme P111A, in 20 mM Tris acetate, pH 8.0, at 25°C Homo sapiens
2400
-
sulfite wild type enzyme, in 20 mM Tris acetate, pH 8.0, at 25°C Homo sapiens
4100
-
sulfite tether deletion mutant enzyme DELTAKVATV, in 20 mM Tris acetate, pH 8.0, at 25°C Homo sapiens
6100
-
sulfite mutant enzyme P105A, in 20 mM Tris acetate, pH 8.0, at 25°C Homo sapiens