Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli strain DH5alpha | Starkeya novella |
Crystallization (Comment) | Organism |
---|---|
- |
Starkeya novella |
Protein Variants | Comment | Organism |
---|---|---|
Y236F | reduced turnover rates and substrate affinity as well as an altered reactivity toward molecular oxygen as an electron acceptor, unlike the wild type enzyme the mutant enzyme is reoxidized quickly in the presence of molecular oxygen | Starkeya novella |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Molybdenum | - |
Starkeya novella |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
42000 | - |
wild type enzyme, analytical ultracentrifugation | Starkeya novella |
43000 | - |
mutant enzyme Y236F, analytical ultracentrifugation | Starkeya novella |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Starkeya novella | - |
- |
- |
Purification (Comment) | Organism |
---|---|
DEAE anion exchange column chromatography | Starkeya novella |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
sulfite + ferricytochrome c + H2O | - |
Starkeya novella | sulfate + ferrocytochrome c + H+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
heterodimer | - |
Starkeya novella |
Synonyms | Comment | Organism |
---|---|---|
SDH | - |
Starkeya novella |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.4 | - |
sulfite | mutant enzyme Y236F, in 20 mM Tris-acetate buffer pH 8.0 | Starkeya novella | |
0.8 | - |
sulfite | mutant enzyme Y236F, in 20 mM bis-Tris-acetate buffer pH 6.0 | Starkeya novella | |
33.51 | - |
sulfite | wild type enzyme, in 20 mM bis-Tris-acetate buffer pH 6.0 | Starkeya novella | |
36.19 | - |
sulfite | mutant enzyme Y236F, in 20 mM bis-Tris-acetate buffer pH 6.0 | Starkeya novella | |
52.51 | - |
sulfite | mutant enzyme Y236F, in 20 mM Tris-acetate buffer pH 8.0 | Starkeya novella | |
333.7 | - |
sulfite | wild type enzyme, in 20 mM Tris-acetate buffer pH 8.0 | Starkeya novella |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5.5 | 9.4 | wild type enzyme | Starkeya novella |
7.2 | 8.64 | mutant enzyme Y236F | Starkeya novella |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
heme c | - |
Starkeya novella |