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Literature summary for 1.8.2.1 extracted from
- The G473D mutation impairs dimerization and catalysis in human sulfite oxidase (2006), Biochemistry, 45, 2149-2160.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli strain TP1000 | Homo sapiens |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| G473A | mutant is able to dimerize and has steady-state activity comparable to that of the wild type, stopped-flow analysis of the reductive half-reaction of this variant yields a rate constant nearly 3 times higher than that of the wild type | Homo sapiens |
| G473D | monomer, mutant is severely impaired both in the ability to bind sulfite and in catalysis, with a second-order rate constant 5 orders of magnitude lower than that of the wild type, significant random-coil formation | Homo sapiens |
| G473W | monomer, mutant with 5fold higher activity than G473D and nearly wild-type activity at pH 7.0 when ferricyanide is the electron acceptor, significant random-coil formation | Homo sapiens |
| R212A/G473D | mutant is able to oligomerize but has undetectable activity, significant random-coil formation | Homo sapiens |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.00456 | - |
sulfite | wild type enzyme, pH 7.0, using ferricyanide as the electron acceptor | Homo sapiens |  |
| 0.00605 | - |
sulfite | mutant enzyme G473A, pH 7.0, using ferricyanide as the electron acceptor | Homo sapiens | |
| 0.0149 | - |
sulfite | wild type enzyme, pH 8.6, using ferricyanide as the electron acceptor | Homo sapiens | |
| 0.191 | - |
sulfite | mutant enzyme G473A, pH 8.5, using ferricyanide as the electron acceptor | Homo sapiens | |
| 21.2 | - |
sulfite | mutant enzyme G473W, pH 7.0, using ferricyanide as the electron acceptor | Homo sapiens | |
| 24 | - |
sulfite | mutant enzyme G473W, pH 8.5, using ferricyanide as the electron acceptor | Homo sapiens | |
| 26.5 | - |
sulfite | mutant enzyme G473D, pH 7.0, using ferricyanide as the electron acceptor | Homo sapiens | |
| 41.4 | - |
sulfite | mutant enzyme G473D, pH 8.5, using ferricyanide as the electron acceptor | Homo sapiens | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mo | consists of a single molybdenum atom coordinated through the dithiolene group of a single molybdopterin molecule | Homo sapiens | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 52000 | - |
1 * 52000, mutant G473D, calculation from amino acid sequence | Homo sapiens |
| 52000 | - |
2 * 52000, wild type enzyme, sedimentation equilibrium analysis | Homo sapiens |
| 53500 | - |
1 * 53500, mutant G473D, gel filtration and sedimentation equilibrium analysis | Homo sapiens |
| 54500 | - |
1 * 54500, mutant G473W, sedimentation equilibrium analysis | Homo sapiens |
| 66600 | - |
1 * 66600, double mutant R212A/G473D, sedimentation equilibrium analysis | Homo sapiens |
| 101000 | - |
wild type enzyme, sedimentation equilibrium analysis | Homo sapiens |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| phenyl-Sepharose column chromatography and Superdex 200 16/60 column gel filtration | Homo sapiens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| sulfite + ferricyanide + H2O | - |
Homo sapiens | sulfate + ferrocyanide | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 52000, wild type enzyme, sedimentation equilibrium analysis | Homo sapiens |
| monomer | 1 * 52000, mutant G473D, calculation from amino acid sequence | Homo sapiens |
| monomer | 1 * 53500, mutant G473D, gel filtration and sedimentation equilibrium analysis | Homo sapiens |
| monomer | 1 * 54500, mutant G473W, sedimentation equilibrium analysis | Homo sapiens |
| monomer | 1 * 66600, double mutant R212A/G473D, sedimentation equilibrium analysis | Homo sapiens |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| SO | - |
Homo sapiens |
| sulfite oxidase | - |
Homo sapiens |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
- |
Homo sapiens |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 10 | - |
Homo sapiens |
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