General Stability | Organism |
---|---|
thawing and refreezing has no effect | Thiobacillus thioparus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
1,10-phenanthroline | 5 mM, complete inhibition | Thiobacillus thioparus | |
2,2'-bipyridyl | 5 mM, complete inhibition | Thiobacillus thioparus | |
AMP | - |
Thiobacillus thioparus | |
arsenite | 1 mM, 83% inhibition | Thiobacillus thioparus | |
Cl- | non-competitive | Thiobacillus thioparus | |
EDTA | 4 mM complete inhibition | Thiobacillus thioparus | |
HgCl2 | 10 mM, complete inhibition | Thiobacillus thioparus | |
KCl | - |
Thiobacillus thioparus | |
NaCl | noncompetitive | Thiobacillus thioparus | |
NEM | 1 mM, 32% inhibition | Thiobacillus thioparus | |
PCMB | 1 mM, complete inhibition | Thiobacillus thioparus | |
phosphate | 50 mM, 90% inhibition, uncompetitive with respect to sulfite | Thiobacillus thioparus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.013 | - |
ferricytochrome c | - |
Thiobacillus thioparus | |
0.088 | - |
sulfite | - |
Thiobacillus thioparus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Iron | enzyme contains nonheme iron | Thiobacillus thioparus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
54000 | - |
sucrose density gradient method | Thiobacillus thioparus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thiobacillus thioparus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Thiobacillus thioparus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.69 | - |
- |
Thiobacillus thioparus |
Storage Stability | Organism |
---|---|
-20°C, stable for several months | Thiobacillus thioparus |
4°C, 10-20% loss of activity after 6 h at room temperature, 80% loss of activity after 5 days | Thiobacillus thioparus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
sulfite + ferricyanide + H2O | - |
Thiobacillus thioparus | sulfate + ferrocyanide + H+ | - |
? | |
sulfite + ferricytochrome c + H2O | horse heart cytochrome or yeast cytochrome | Thiobacillus thioparus | sulfate + ferrocytochrome c + H+ | - |
? |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
55 | - |
9 min, 95% loss of activity | Thiobacillus thioparus |
60 | - |
6 min, 95% loss of activity | Thiobacillus thioparus |
100 | - |
1 min, complete loss of activity | Thiobacillus thioparus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
with Tricine-HCl or Tricine-NaOH buffer | Thiobacillus thioparus |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
38 | - |
Cl- | - |
Thiobacillus thioparus |