Application | Comment | Organism |
---|---|---|
medicine | the enzyme is a promising target for anti Fasciola treatments | Fasciola hepatica |
Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21 (DE3) cells | Fasciola hepatica |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
66000 | - |
x * 66000, SDS-PAGE | Fasciola hepatica |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
GSSG + NADPH + H+ | Fasciola hepatica | - |
GSH + NADP+ | - |
? | |
thioredoxin disulfide + NADPH + H+ | Fasciola hepatica | - |
thioredoxin + NADP+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Fasciola hepatica | A8E0R8 | - |
- |
Purification (Comment) | Organism |
---|---|
ammonium sulfate precipitation, Ni-NTA column chromatography, GSH-Sepharose column chromatography, and 2',5'-ADP-Sepharose column chromatography | Fasciola hepatica |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
5,5'-dithiobis(2-nitrobenzoic acid) + NADPH + H+ | - |
Fasciola hepatica | 2-nitro-5-thiobenzoate + NADP+ | - |
? | |
GSSG + NADPH + H+ | - |
Fasciola hepatica | GSH + NADP+ | - |
? | |
thioredoxin disulfide + NADPH + H+ | - |
Fasciola hepatica | thioredoxin + NADP+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 66000, SDS-PAGE | Fasciola hepatica |
Synonyms | Comment | Organism |
---|---|---|
TGR | - |
Fasciola hepatica |
thioredoxin-glutathione reductase | - |
Fasciola hepatica |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | the FAD content of the recombinant enzyme is approximately 25% | Fasciola hepatica | |
NADPH | - |
Fasciola hepatica |