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Literature summary for 1.8.1.9 extracted from
- A hyperthermostable novel protein-disulfide oxidoreductase is reduced by thioredoxin reductase from hyperthermophilic archaeon Pyrococcus horikoshii (2003), Arch. Biochem. Biophys., 418, 179-185 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli Rosetta(DE3) | Pyrococcus furiosus |
| expression in Escherichia coli Rosetta(DE3) | Pyrococcus horikoshii |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| C146S | no activity against insulin disulfide, no DTNB-reducing activity | Pyrococcus furiosus |
| C35S | strong activity as high as that of a wild type against insulin disulfide, 98% of residual activity. The activity of C35S against the reduction of DTNB was not decreased | Pyrococcus furiosus |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 27000 | - |
gel filtration | Pyrococcus furiosus |
| 70000 | - |
gel filtration | Pyrococcus horikoshii |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| protein-disulfide + NADPH + H+ | Pyrococcus furiosus | the enzyme is involved in a thioredoxin like system with thioredoxin reductase (PH1426). The redox potential of the redox protein is similar to that of thioredoxin from Escherichia coli and lower than that of glutathione | protein-dithiol + NADP+ | - |
? |  |
| protein-disulfide + NADPH + H+ | Pyrococcus furiosus OT-3 | the enzyme is involved in a thioredoxin like system with thioredoxin reductase (PH1426). The redox potential of the redox protein is similar to that of thioredoxin from Escherichia coli and lower than that of glutathione | protein-dithiol + NADP+ | - |
? | |
| thioredoxin + NADP+ | Pyrococcus horikoshii | the enzyme forms a redox system with protein-disulfide oxidoreductase PhRP (PH0178) | thioredoxin disulfide + NADPH + H+ | - |
? | |
| thioredoxin + NADP+ | Pyrococcus horikoshii DSM 12428 | the enzyme forms a redox system with protein-disulfide oxidoreductase PhRP (PH0178) | thioredoxin disulfide + NADPH + H+ | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pyrococcus furiosus | O57917 | - |
- |
| Pyrococcus furiosus OT-3 | O57917 | - |
- |
| Pyrococcus horikoshii | O50134 | - |
- |
| Pyrococcus horikoshii DSM 12428 | O50134 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Pyrococcus furiosus |
- |
Pyrococcus horikoshii |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | the enzyme catalyzes the reduction of protein-disulfide oxidoreductase PhRP (PH0178) | Pyrococcus horikoshii | ? | - |
? | |
| additional information | the enzyme catalyzes the reduction of protein-disulfide oxidoreductase PhRP (PH0178) | Pyrococcus horikoshii DSM 12428 | ? | - |
? | |
| protein-disulfide + NADH + H+ | reduction activity for the disulfides in bovine insulin | Pyrococcus furiosus | protein-dithiol + NAD+ | - |
? | |
| protein-disulfide + NADH + H+ | reduction activity for the disulfides in bovine insulin | Pyrococcus furiosus OT-3 | protein-dithiol + NAD+ | - |
? | |
| protein-disulfide + NADPH + H+ | the enzyme is involved in a thioredoxin like system with thioredoxin reductase (PH1426). The redox potential of the redox protein is similar to that of thioredoxin from Escherichia coli and lower than that of glutathione | Pyrococcus furiosus | protein-dithiol + NADP+ | - |
? | |
| protein-disulfide + NADPH + H+ | reduction activity for the disulfides in bovine insulin | Pyrococcus furiosus | protein-dithiol + NADP+ | - |
? | |
| protein-disulfide + NADPH + H+ | the enzyme is involved in a thioredoxin like system with thioredoxin reductase (PH1426). The redox potential of the redox protein is similar to that of thioredoxin from Escherichia coli and lower than that of glutathione | Pyrococcus furiosus OT-3 | protein-dithiol + NADP+ | - |
? | |
| protein-disulfide + NADPH + H+ | reduction activity for the disulfides in bovine insulin | Pyrococcus furiosus OT-3 | protein-dithiol + NADP+ | - |
? | |
| thioredoxin + NADP+ | - |
Pyrococcus horikoshii | thioredoxin disulfide + NADPH + H+ | - |
? | |
| thioredoxin + NADP+ | the enzyme forms a redox system with protein-disulfide oxidoreductase PhRP (PH0178) | Pyrococcus horikoshii | thioredoxin disulfide + NADPH + H+ | - |
? | |
| thioredoxin + NADP+ | - |
Pyrococcus horikoshii DSM 12428 | thioredoxin disulfide + NADPH + H+ | - |
? | |
| thioredoxin + NADP+ | the enzyme forms a redox system with protein-disulfide oxidoreductase PhRP (PH0178) | Pyrococcus horikoshii DSM 12428 | thioredoxin disulfide + NADPH + H+ | - |
? | |
| [redox protein PhRP]-disulfide + NADH + H+ | redox protein PhRP from PH0178 | Pyrococcus furiosus | [redox protein PhRP]-dithiol + NAD+ | - |
? | |
| [redox protein PhRP]-disulfide + NADH + H+ | redox protein PhRP from PH0178 | Pyrococcus furiosus OT-3 | [redox protein PhRP]-dithiol + NAD+ | - |
? | |
| [redox protein PhRP]-disulfide + NADPH + H+ | redox protein PhRP from PH0178 | Pyrococcus furiosus | [redox protein PhRP]-dithiol + NADP+ | - |
? | |
| [redox protein PhRP]-disulfide + NADPH + H+ | redox protein PhRP from PH0178 | Pyrococcus furiosus OT-3 | [redox protein PhRP]-dithiol + NADP+ | - |
? | |
| [thioredoxin reductase from Escherichia coli]-disulfide + NADH + H+ | - |
Pyrococcus furiosus | [thioredoxin reductase from Escherichia coli]-dithiol + NAD+ | - |
? | |
| [thioredoxin reductase from Escherichia coli]-disulfide + NADPH + H+ | - |
Pyrococcus furiosus | [thioredoxin reductase from Escherichia coli]-dithiol + NADP+ | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| homodimer | 2 * 36000, SDS-PAGE | Pyrococcus horikoshii |
| monomer | 1 * 26000, SDS-PAGE | Pyrococcus furiosus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| PH0178 | - |
Pyrococcus furiosus |
| PH1426 | - |
Pyrococcus horikoshii |
| PhRP | - |
Pyrococcus furiosus |
| PhTrxR | - |
Pyrococcus horikoshii |
| protein-disulfide oxidoreductase | - |
Pyrococcus furiosus |
| thioredoxin reductase | - |
Pyrococcus horikoshii |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
assay at | Pyrococcus furiosus |
| 25 | - |
assay at | Pyrococcus horikoshii |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 100 | - |
retains full activity for over 1 h at 100°C | Pyrococcus furiosus |
| 100 | - |
retains full activity for over 1 h at 100°C | Pyrococcus horikoshii |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| flavin | in the sequence, PhTrxR appears to be the subfamily of flavoprotein reductase flavoprotein | Pyrococcus horikoshii | |
| NADP+ | - |
Pyrococcus furiosus | |
| NADP+ | - |
Pyrococcus horikoshii | |
| NADPH | - |
Pyrococcus furiosus | |
General Information
| General Information | Comment | Organism |
|---|---|---|
| metabolism | the enzyme is involved in a thioredoxin like system with thioredoxin reductase (PH1426). The redox potential of the redox protein is similar to that of thioredoxin from Escherichia coli and lower than that of glutathione | Pyrococcus furiosus |
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