Cloned (Comment) | Organism |
---|---|
- |
Arabidopsis thaliana |
- |
Hordeum vulgare subsp. vulgare |
Crystallization (Comment) | Organism |
---|---|
molecular modelling of the complex with thioredoxin | Hordeum vulgare subsp. vulgare |
Protein Variants | Comment | Organism |
---|---|---|
C145S | active site cysteine residue. Kinetic data | Hordeum vulgare subsp. vulgare |
C148S | residue Cys148 probably performs an initial nucleophilic attack on the active site disulfide in thioredoxin disulfide. Kinetic data | Hordeum vulgare subsp. vulgare |
Delta42-47 | FAD-domain mutant. Kinetic data | Hordeum vulgare subsp. vulgare |
G222D/A223G/G224E | NADPH-domain mutant. Kinetic data | Hordeum vulgare subsp. vulgare |
G225R/G226D | NADPH-domain mutant. Kinetic data | Hordeum vulgare subsp. vulgare |
G225R/G226D/P227V | NADPH-domain mutant. Kinetic data | Hordeum vulgare subsp. vulgare |
M43A | mutant in a loop of the FAD-binding domain, strongly affects the interaction with thioredoxin. Kinetic data | Hordeum vulgare subsp. vulgare |
N139A | NADPH-domain mutant. Kinetic data | Hordeum vulgare subsp. vulgare |
N45A/D46A | FAD-domain mutant. Kinetic data | Hordeum vulgare subsp. vulgare |
R140A | NADPH-domain mutant. Kinetic data | Hordeum vulgare subsp. vulgare |
W42A | mutant in a loop of the FAD-binding domain, strongly affects the interaction with thioredoxin. Kinetic data | Hordeum vulgare subsp. vulgare |
W42A/M43A | mutant in a loop of the FAD-binding domain, strongly affects the interaction with thioredoxin | Hordeum vulgare subsp. vulgare |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00041 | - |
Hordeum vulgare thioredoxin 2 mutant E86A | mutant N139A, pH 7.5, temperature not specified in the publication | Hordeum vulgare subsp. vulgare | |
0.00048 | - |
Arabidopsis thaliana thioredoxin 3 | pH 7.5, temperature not specified in the publication | Arabidopsis thaliana | |
0.00052 | - |
Arabidopsis thaliana thioredoxin 3 | mutant G222D/A223G/G224E, pH 7.5, temperature not specified in the publication | Hordeum vulgare subsp. vulgare | |
0.00057 | - |
Hordeum vulgare thioredoxin 2 mutant E86A | wild-type, pH 7.5, temperature not specified in the publication | Hordeum vulgare subsp. vulgare | |
0.00067 | - |
Arabidopsis thaliana thioredoxin 3 | mutant G225R/G226D, pH 7.5, temperature not specified in the publication | Hordeum vulgare subsp. vulgare | |
0.00068 | - |
Hordeum vulgare thioredoxin 2 | mutant N139A, pH 7.5, temperature not specified in the publication | Hordeum vulgare subsp. vulgare | |
0.00073 | - |
Arabidopsis thaliana thioredoxin 3 | mutant G225R/G226D/P227V, pH 7.5, temperature not specified in the publication | Hordeum vulgare subsp. vulgare | |
0.0008 | - |
Arabidopsis thaliana thioredoxin 3 | wild-type, pH 7.5, temperature not specified in the publication | Hordeum vulgare subsp. vulgare | |
0.00105 | - |
Hordeum vulgare thioredoxin 2 | mutant G225R/G226D/P227V, pH 7.5, temperature not specified in the publication | Hordeum vulgare subsp. vulgare | |
0.00117 | - |
Hordeum vulgare thioredoxin 2 | wild-type, pH 7.5, temperature not specified in the publication | Hordeum vulgare subsp. vulgare | |
0.00123 | - |
Hordeum vulgare thioredoxin 1 | wild-type, pH 7.5, temperature not specified in the publication | Hordeum vulgare subsp. vulgare | |
0.00128 | - |
Hordeum vulgare thioredoxin 2 | mutant G225R/G226D, pH 7.5, temperature not specified in the publication | Hordeum vulgare subsp. vulgare | |
0.0014 | - |
Hordeum vulgare thioredoxin 2 mutant G57P | wild-type, pH 7.5, temperature not specified in the publication | Hordeum vulgare subsp. vulgare | |
0.00146 | - |
Hordeum vulgare thioredoxin 2 | mutant G222D/A223G/G224E, pH 7.5, temperature not specified in the publication | Hordeum vulgare subsp. vulgare | |
0.0016 | - |
Hordeum vulgare thioredoxin 2 mutant E86A | mutant R140A, pH 7.5, temperature not specified in the publication | Hordeum vulgare subsp. vulgare | |
0.0022 | - |
Hordeum vulgare thioredoxin 2 | mutant N45A/D46A, pH 7.5, temperature not specified in the publication | Hordeum vulgare subsp. vulgare | |
0.0024 | - |
Arabidopsis thaliana thioredoxin 3 | mutant W42A, pH 7.5, temperature not specified in the publication | Hordeum vulgare subsp. vulgare | |
0.00343 | - |
Hordeum vulgare thioredoxin 2 | mutant R140A, pH 7.5, temperature not specified in the publication | Hordeum vulgare subsp. vulgare | |
0.0042 | - |
Arabidopsis thaliana thioredoxin 3 | mutant N45A/D46A, pH 7.5, temperature not specified in the publication | Hordeum vulgare subsp. vulgare | |
0.0141 | - |
Arabidopsis thaliana thioredoxin 3 | mutant M43A, pH 7.5, temperature not specified in the publication | Hordeum vulgare subsp. vulgare | |
0.0155 | - |
Hordeum vulgare thioredoxin 2 mutant I51G | wild-type, pH 7.5, temperature not specified in the publication | Hordeum vulgare subsp. vulgare | |
0.016 | - |
Hordeum vulgare thioredoxin 2 | mutant M43A, pH 7.5, temperature not specified in the publication | Hordeum vulgare subsp. vulgare | |
0.0305 | - |
Arabidopsis thaliana thioredoxin 3 | mutant W42A/M43A, pH 7.5, temperature not specified in the publication | Hordeum vulgare subsp. vulgare | |
0.0457 | - |
Hordeum vulgare thioredoxin 2 | mutant W42A, pH 7.5, temperature not specified in the publication | Hordeum vulgare subsp. vulgare | |
0.0543 | - |
Arabidopsis thaliana thioredoxin 3 | mutant Delta42-47, pH 7.5, temperature not specified in the publication | Hordeum vulgare subsp. vulgare | |
0.06 | - |
Hordeum vulgare thioredoxin 2 | pH 7.5, temperature not specified in the publication | Arabidopsis thaliana | |
0.149 | - |
Escherichia coli thioredoxin | wild-type, pH 7.5, temperature not specified in the publication | Hordeum vulgare subsp. vulgare |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
mitochondrion | - |
Arabidopsis thaliana | 5739 | - |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | Q39243 | - |
- |
Hordeum vulgare subsp. vulgare | A9LN30 | isoform Ntr2 | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Arabidopsis thaliana thioredoxin 3 + NADP+ | - |
Arabidopsis thaliana | Arabidopsis thaliana thioredoxin 3 disulfide + NADPH + H+ | - |
? | |
Arabidopsis thaliana thioredoxin 3 + NADP+ | - |
Hordeum vulgare subsp. vulgare | Arabidopsis thaliana thioredoxin 3 disulfide + NADPH + H+ | - |
? | |
Escherichia coli thioredoxin + NADP+ | - |
Arabidopsis thaliana | Escherichia coli thioredoxin disulfide + NADPH + H+ | - |
? | |
Escherichia coli thioredoxin + NADP+ | - |
Hordeum vulgare subsp. vulgare | Escherichia coli thioredoxin disulfide + NADPH + H+ | - |
? | |
Hordeum vulgare thioredoxin 1 + NADP+ | - |
Hordeum vulgare subsp. vulgare | Hordeum vulgare thioredoxin 1 disulfide + NADPH + H+ | - |
? | |
Hordeum vulgare thioredoxin 2 + NADP+ | - |
Arabidopsis thaliana | Hordeum vulgare thioredoxin 2 disulfide + NADPH + H+ | - |
? | |
Hordeum vulgare thioredoxin 2 + NADP+ | - |
Hordeum vulgare subsp. vulgare | Hordeum vulgare thioredoxin 2 disulfide + NADPH + H+ | - |
? | |
Hordeum vulgare thioredoxin 2 mutant E86A + NADP+ | - |
Hordeum vulgare subsp. vulgare | Hordeum vulgare thioredoxin 2 mutant E86A disulfide + NADPH + H+ | - |
? | |
Hordeum vulgare thioredoxin 2 mutant G47P + NADP+ | - |
Arabidopsis thaliana | Hordeum vulgare thioredoxin 2 mutant G47P disulfide + NADPH + H+ | - |
? | |
Hordeum vulgare thioredoxin 2 mutant G57P + NADP+ | - |
Hordeum vulgare subsp. vulgare | Hordeum vulgare thioredoxin 2 mutant G57P disulfide + NADPH + H+ | - |
? | |
Hordeum vulgare thioredoxin 2 mutant I51G + NADP+ | - |
Arabidopsis thaliana | Hordeum vulgare thioredoxin 2 mutant I51G disulfide + NADPH + H+ | - |
? | |
Hordeum vulgare thioredoxin 2 mutant I51G + NADP+ | - |
Hordeum vulgare subsp. vulgare | Hordeum vulgare thioredoxin 2 mutant I51G disulfide + NADPH + H+ | - |
? | |
additional information | binding of NADP+ enhances the ability of Ntr2 to bind thioredoxin | Hordeum vulgare subsp. vulgare | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
NTR2 | - |
Hordeum vulgare subsp. vulgare |
NtrB | - |
Arabidopsis thaliana |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.8 | - |
Hordeum vulgare thioredoxin 2 | mutant G225R/G226D/P227V, pH 7.5, temperature not specified in the publication | Hordeum vulgare subsp. vulgare | |
2.6 | - |
Hordeum vulgare thioredoxin 2 mutant E86A | mutant R140A, pH 7.5, temperature not specified in the publication | Hordeum vulgare subsp. vulgare | |
3.9 | - |
Arabidopsis thaliana thioredoxin 3 | mutant G225R/G226D/P227V, pH 7.5, temperature not specified in the publication | Hordeum vulgare subsp. vulgare | |
4.2 | - |
Hordeum vulgare thioredoxin 2 | mutant N139A, pH 7.5, temperature not specified in the publication | Hordeum vulgare subsp. vulgare | |
4.2 | - |
Hordeum vulgare thioredoxin 2 mutant E86A | mutant N139A, pH 7.5, temperature not specified in the publication | Hordeum vulgare subsp. vulgare | |
4.8 | - |
Arabidopsis thaliana thioredoxin 3 | wild-type, pH 7.5, temperature not specified in the publication | Hordeum vulgare subsp. vulgare | |
5 | - |
Arabidopsis thaliana thioredoxin 3 | mutant G222D/A223G/G224E, pH 7.5, temperature not specified in the publication | Hordeum vulgare subsp. vulgare | |
5 | - |
Arabidopsis thaliana thioredoxin 3 | mutant G225R/G226D, pH 7.5, temperature not specified in the publication | Hordeum vulgare subsp. vulgare | |
5.1 | - |
Hordeum vulgare thioredoxin 2 | mutant R140A, pH 7.5, temperature not specified in the publication | Hordeum vulgare subsp. vulgare | |
5.3 | - |
Hordeum vulgare thioredoxin 2 mutant E86A | wild-type, pH 7.5, temperature not specified in the publication | Hordeum vulgare subsp. vulgare | |
5.9 | - |
Arabidopsis thaliana thioredoxin 3 | pH 7.5, temperature not specified in the publication | Arabidopsis thaliana | |
7.3 | - |
Hordeum vulgare thioredoxin 2 | mutant N45A/D46A, pH 7.5, temperature not specified in the publication | Hordeum vulgare subsp. vulgare | |
7.9 | - |
Hordeum vulgare thioredoxin 2 mutant G57P | wild-type, pH 7.5, temperature not specified in the publication | Hordeum vulgare subsp. vulgare | |
7.9 | - |
Hordeum vulgare thioredoxin 2 | mutant G222D/A223G/G224E, pH 7.5, temperature not specified in the publication | Hordeum vulgare subsp. vulgare | |
8 | - |
Hordeum vulgare thioredoxin 2 | wild-type, pH 7.5, temperature not specified in the publication | Hordeum vulgare subsp. vulgare | |
8.1 | - |
Hordeum vulgare thioredoxin 2 | mutant G225R/G226D, pH 7.5, temperature not specified in the publication | Hordeum vulgare subsp. vulgare | |
8.5 | - |
Hordeum vulgare thioredoxin 2 | pH 7.5, temperature not specified in the publication | Arabidopsis thaliana | |
9.4 | - |
Escherichia coli thioredoxin | wild-type, pH 7.5, temperature not specified in the publication | Hordeum vulgare subsp. vulgare | |
10.4 | - |
Hordeum vulgare thioredoxin 2 | mutant W42A, pH 7.5, temperature not specified in the publication | Hordeum vulgare subsp. vulgare | |
10.4 | - |
Arabidopsis thaliana thioredoxin 3 | mutant W42A, pH 7.5, temperature not specified in the publication | Hordeum vulgare subsp. vulgare | |
10.7 | - |
Arabidopsis thaliana thioredoxin 3 | mutant N45A/D46A, pH 7.5, temperature not specified in the publication | Hordeum vulgare subsp. vulgare | |
11 | - |
Arabidopsis thaliana thioredoxin 3 | mutant W42A/M43A, pH 7.5, temperature not specified in the publication | Hordeum vulgare subsp. vulgare | |
11.4 | - |
Hordeum vulgare thioredoxin 2 | mutant M43A, pH 7.5, temperature not specified in the publication | Hordeum vulgare subsp. vulgare | |
12.1 | - |
Hordeum vulgare thioredoxin 1 | wild-type, pH 7.5, temperature not specified in the publication | Hordeum vulgare subsp. vulgare | |
14.8 | - |
Arabidopsis thaliana thioredoxin 3 | mutant M43A, pH 7.5, temperature not specified in the publication | Hordeum vulgare subsp. vulgare | |
15.3 | - |
Arabidopsis thaliana thioredoxin 3 | mutant Delta42-47, pH 7.5, temperature not specified in the publication | Hordeum vulgare subsp. vulgare | |
15.9 | - |
Hordeum vulgare thioredoxin 2 mutant I51G | wild-type, pH 7.5, temperature not specified in the publication | Hordeum vulgare subsp. vulgare |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | - |
Arabidopsis thaliana | |
FAD | - |
Hordeum vulgare subsp. vulgare | |
NADP+ | - |
Arabidopsis thaliana | |
NADP+ | - |
Hordeum vulgare subsp. vulgare |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
63.1 | - |
Escherichia coli thioredoxin | wild-type, pH 7.5, temperature not specified in the publication | Hordeum vulgare subsp. vulgare | |
140 | - |
Hordeum vulgare thioredoxin 2 | pH 7.5, temperature not specified in the publication | Arabidopsis thaliana | |
228 | - |
Hordeum vulgare thioredoxin 2 | mutant W42A, pH 7.5, temperature not specified in the publication | Hordeum vulgare subsp. vulgare | |
282 | - |
Arabidopsis thaliana thioredoxin 3 | mutant Delta42-47, pH 7.5, temperature not specified in the publication | Hordeum vulgare subsp. vulgare | |
361 | - |
Arabidopsis thaliana thioredoxin 3 | mutant W42A/M43A, pH 7.5, temperature not specified in the publication | Hordeum vulgare subsp. vulgare | |
713 | - |
Hordeum vulgare thioredoxin 2 | mutant M43A, pH 7.5, temperature not specified in the publication | Hordeum vulgare subsp. vulgare | |
1030 | - |
Hordeum vulgare thioredoxin 2 mutant I51G | wild-type, pH 7.5, temperature not specified in the publication | Hordeum vulgare subsp. vulgare | |
1050 | - |
Arabidopsis thaliana thioredoxin 3 | mutant M43A, pH 7.5, temperature not specified in the publication | Hordeum vulgare subsp. vulgare | |
1490 | - |
Hordeum vulgare thioredoxin 2 | mutant R140A, pH 7.5, temperature not specified in the publication | Hordeum vulgare subsp. vulgare | |
1630 | - |
Hordeum vulgare thioredoxin 2 mutant E86A | mutant R140A, pH 7.5, temperature not specified in the publication | Hordeum vulgare subsp. vulgare | |
1710 | - |
Hordeum vulgare thioredoxin 2 | mutant G225R/G226D/P227V, pH 7.5, temperature not specified in the publication | Hordeum vulgare subsp. vulgare | |
2550 | - |
Arabidopsis thaliana thioredoxin 3 | mutant N45A/D46A, pH 7.5, temperature not specified in the publication | Hordeum vulgare subsp. vulgare | |
3300 | - |
Hordeum vulgare thioredoxin 2 | mutant N45A/D46A, pH 7.5, temperature not specified in the publication | Hordeum vulgare subsp. vulgare | |
4240 | - |
Arabidopsis thaliana thioredoxin 3 | mutant W42A, pH 7.5, temperature not specified in the publication | Hordeum vulgare subsp. vulgare | |
5340 | - |
Arabidopsis thaliana thioredoxin 3 | mutant G225R/G226D/P227V, pH 7.5, temperature not specified in the publication | Hordeum vulgare subsp. vulgare | |
5410 | - |
Hordeum vulgare thioredoxin 2 | mutant G222D/A223G/G224E, pH 7.5, temperature not specified in the publication | Hordeum vulgare subsp. vulgare | |
5490 | - |
Hordeum vulgare thioredoxin 2 mutant G57P | wild-type, pH 7.5, temperature not specified in the publication | Hordeum vulgare subsp. vulgare | |
6000 | - |
Arabidopsis thaliana thioredoxin 3 | wild-type, pH 7.5, temperature not specified in the publication | Hordeum vulgare subsp. vulgare | |
6180 | - |
Hordeum vulgare thioredoxin 2 | mutant N139A, pH 7.5, temperature not specified in the publication | Hordeum vulgare subsp. vulgare | |
6330 | - |
Hordeum vulgare thioredoxin 2 | mutant G225R/G226D, pH 7.5, temperature not specified in the publication | Hordeum vulgare subsp. vulgare | |
6800 | - |
Hordeum vulgare thioredoxin 2 | wild-type, pH 7.5, temperature not specified in the publication | Hordeum vulgare subsp. vulgare | |
7460 | - |
Arabidopsis thaliana thioredoxin 3 | mutant G225R/G226D, pH 7.5, temperature not specified in the publication | Hordeum vulgare subsp. vulgare | |
9230 | - |
Hordeum vulgare thioredoxin 2 mutant E86A | wild-type, pH 7.5, temperature not specified in the publication | Hordeum vulgare subsp. vulgare | |
9620 | - |
Arabidopsis thaliana thioredoxin 3 | mutant G222D/A223G/G224E, pH 7.5, temperature not specified in the publication | Hordeum vulgare subsp. vulgare | |
9840 | - |
Hordeum vulgare thioredoxin 1 | wild-type, pH 7.5, temperature not specified in the publication | Hordeum vulgare subsp. vulgare | |
10200 | - |
Hordeum vulgare thioredoxin 2 mutant E86A | mutant N139A, pH 7.5, temperature not specified in the publication | Hordeum vulgare subsp. vulgare | |
12300 | - |
Arabidopsis thaliana thioredoxin 3 | pH 7.5, temperature not specified in the publication | Arabidopsis thaliana |