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Literature summary for 1.8.1.9 extracted from

  • Regner, E.L.; Thompson, C.S.; Iglesias, A.A.; Guerrero, S.A.; Arias, D.G.
    Biochemical characterization of thioredoxin reductase from Babesia bovis (2014), Biochimie, 99, 44-53 .
    View publication on PubMed
Search for more literature for 1.8.1.9

Cloned(Commentary)

Cloned (Comment) Organism
-
 Babesia bovis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0013
-
 NADPH pH 8.0, 30°C Babesia bovis
0.0042
-
 Babesia bovis thioredoxin pH 8.0, 30°C Babesia bovis
0.007
-
 Escherichia coli thioredoxin pH 8.0, 30°C Babesia bovis
0.238
-
 5,5'-dithio-bis(2-nitrobenzoic acid) pH 8.0, 30°C Babesia bovis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
100000
-
 gel filtration Babesia bovis

Organism

Organism UniProt Comment Textmining
Babesia bovis A7AW73
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5,5'-dithio-bis(2-nitrobenzoic acid) + NADPH + H+
-
 Babesia bovis 5'-thionitrobenzoic acid + NADP+
-
 ?
Babesia bovis thioredoxin + NADP+
-
 Babesia bovis Babesia bovis thioredoxin disulfide + NADPH + H+
-
 ?
Escherichia coli thioredoxin + NADP+
-
 Babesia bovis Escherichia coli thioredoxin disulfide + NADPH + H+
-
 ?
additional information the thioredoxin reductase/thioredoxin system can reduce oxidized glutathione and S-nitrosoglutathione Babesia bovis ?
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 60700, calculated Babesia bovis

Synonyms

Synonyms Comment Organism
BBOV_I002190
-
 Babesia bovis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
-
 Babesia bovis

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.1
-
 Escherichia coli thioredoxin pH 8.0, 30°C Babesia bovis
10.8
-
 5,5'-dithio-bis(2-nitrobenzoic acid) pH 8.0, 30°C Babesia bovis
20
-
 NADPH pH 8.0, 30°C Babesia bovis
20
-
 Babesia bovis thioredoxin pH 8.0, 30°C Babesia bovis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
 Babesia bovis

Cofactor

Cofactor Comment Organism Structure
FAD absorption spectrum shows maxima at 380 and 460 nm Babesia bovis
additional information no substrate: NADH Babesia bovis
NADPH no substrate inhibition up to 0.1 mM Babesia bovis

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.075
-
 5,5'-dithio-bis(2-nitrobenzoic acid) pH 8.0, 30°C Babesia bovis
10
-
 Escherichia coli thioredoxin pH 8.0, 30°C Babesia bovis
80
-
 Babesia bovis thioredoxin pH 8.0, 30°C Babesia bovis
250
-
 NADPH pH 8.0, 30°C Babesia bovis