Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) or ER2566 cells | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
auranofin | efficient inhibition, about 20% residual activity after 8 h at 0.005 mM, about 7% residual activity after 8 h at 0.05 mM | Homo sapiens | |
aurothioglucose | about 90% residual activity after 8 h at 0.005 mM, about 80% residual activity after 8 h at 0.05 mM | Homo sapiens | |
cisplatin | about 40% residual activity after 8 h at 0.005 mM or 0.05 mM | Homo sapiens | |
[(iPr2Im)2Au]Cl | mainly inhibits isoform TrxR2, about 30% residual activity after 8 h at 0.005 mM or 0.05 mM | Homo sapiens | |
[Au(d2pype)2]Cl | mainly inhibits isoform TrxR1, about 30% residual activity after 8 h at 0.005 mM, about 10% residual activity after 8 h at 0.05 mM | Homo sapiens | |
[Au(d2pypp)2]Cl | about 30% residual activity after 8 h at 0.005 mM, about 10% residual activity after 8 h at 0.05 mM | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0019 | - |
Lipoamide | isoform TrxR1, in 50 mM Tris-HCl, 1 mM EDTA, pH 8.0, at 37°C | Homo sapiens | |
0.0027 | - |
lipoic acid | isoform TrxR1, in 50 mM Tris-HCl, 1 mM EDTA, pH 8.0, at 37°C | Homo sapiens | |
0.0043 | - |
Lipoamide | isoform TrxR2, in 50 mM Tris-HCl, 1 mM EDTA, pH 8.0, at 37°C | Homo sapiens | |
0.105 | - |
5,5'-dithiobis(2-nitrobenzoic acid) | isoform TrxR1, in 50 mM Tris-HCl, 1 mM EDTA, pH 7.0, at 37°C | Homo sapiens | |
0.39 | - |
5,5'-dithiobis(2-nitrobenzoic acid) | isoform TrxR2, in 50 mM Tris-HCl, 1 mM EDTA, pH 7.0, at 37°C | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | isoform TrxR1 | Homo sapiens | 5829 | - |
mitochondrion | isoform TrxR2 | Homo sapiens | 5739 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | selenoprotein | Homo sapiens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
53117 | - |
2 * 53117, isoform TrxR1, calculated from amino acid sequence | Homo sapiens |
54769 | - |
2 * 54769, isoform TrxR2, calculated from amino acid sequence | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
thioredoxin 1 + NADP+ | Homo sapiens | TrxR2 prefers its endogenous substrate thioredocin 2 over thioredoxin 1 (10fold), whereas isoform TrxR1 efficiently reduces both thioredoxin 1 and thioredoxin 2 | thioredoxin 1 disulfide + NADPH + H+ | - |
? | |
thioredoxin 2 + NADP+ | Homo sapiens | TrxR2 prefers its endogenous substrate thioredocin 2 over thioredoxin 1 (10fold), whereas isoform TrxR1 efficiently reduces both thioredoxin 1 and thioredoxin 2 | thioredoxin 2 disulfide + NADPH + H+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
2',5'-ADP Sepharose column chromatography, PAO Sepharose column chromatography, and Superdex 200 gel filtration | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
5,5'-dithiobis(2-nitrobenzoic acid) + NADPH + H+ | isoform TrxR2 displays strikingly lower activity with 5,5'-dithiobis(2-nitrobenzoic acid) compared to isoform TrxR1 | Homo sapiens | 5'-thionitrobenzoic acid + NADP+ | - |
? | |
juglone + NADPH + H+ | isoform TrxR2 displays strikingly lower activity with juglone compared to isoform TrxR1 | Homo sapiens | ? | - |
? | |
lipoamide + NADPH + H+ | isoform TrxR2 displays strikingly lower activity with lipoamide compared to isoform TrxR1 | Homo sapiens | ? | - |
? | |
lipoic acid + NADPH + H+ | substrate for isoform TrxR1 | Homo sapiens | ? | - |
? | |
additional information | isoform TrxR2 cannot reduce lipoic acid | Homo sapiens | ? | - |
? | |
thioredoxin 1 + NADP+ | TrxR2 prefers its endogenous substrate thioredocin 2 over thioredoxin 1 (10fold), whereas isoform TrxR1 efficiently reduces both thioredoxin 1 and thioredoxin 2 | Homo sapiens | thioredoxin 1 disulfide + NADPH + H+ | - |
? | |
thioredoxin 2 + NADP+ | TrxR2 prefers its endogenous substrate thioredocin 2 over thioredoxin 1 (10fold), whereas isoform TrxR1 efficiently reduces both thioredoxin 1 and thioredoxin 2 | Homo sapiens | thioredoxin 2 disulfide + NADPH + H+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | 2 * 53117, isoform TrxR1, calculated from amino acid sequence | Homo sapiens |
homodimer | 2 * 54769, isoform TrxR2, calculated from amino acid sequence | Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
TrxR1 | - |
Homo sapiens |
TrxR2 | - |
Homo sapiens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.6 | - |
Lipoamide | isoform TrxR2, in 50 mM Tris-HCl, 1 mM EDTA, pH 8.0, at 37°C | Homo sapiens | |
1.8 | - |
lipoic acid | isoform TrxR1, in 50 mM Tris-HCl, 1 mM EDTA, pH 8.0, at 37°C | Homo sapiens | |
3.3 | - |
Lipoamide | isoform TrxR1, in 50 mM Tris-HCl, 1 mM EDTA, pH 8.0, at 37°C | Homo sapiens | |
8.28 | - |
5,5'-dithiobis(2-nitrobenzoic acid) | isoform TrxR2, in 50 mM Tris-HCl, 1 mM EDTA, pH 7.0, at 37°C | Homo sapiens | |
18.73 | - |
5,5'-dithiobis(2-nitrobenzoic acid) | isoform TrxR1, in 50 mM Tris-HCl, 1 mM EDTA, pH 7.0, at 37°C | Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | - |
Homo sapiens | |
NADPH | dependent on | Homo sapiens |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
21.2 | - |
5,5'-dithiobis(2-nitrobenzoic acid) | isoform TrxR2, in 50 mM Tris-HCl, 1 mM EDTA, pH 7.0, at 37°C | Homo sapiens | |
178 | - |
5,5'-dithiobis(2-nitrobenzoic acid) | isoform TrxR1, in 50 mM Tris-HCl, 1 mM EDTA, pH 7.0, at 37°C | Homo sapiens | |
372 | - |
Lipoamide | isoform TrxR2, in 50 mM Tris-HCl, 1 mM EDTA, pH 8.0, at 37°C | Homo sapiens | |
667 | - |
lipoic acid | isoform TrxR1, in 50 mM Tris-HCl, 1 mM EDTA, pH 8.0, at 37°C | Homo sapiens | |
1737 | - |
Lipoamide | isoform TrxR1, in 50 mM Tris-HCl, 1 mM EDTA, pH 8.0, at 37°C | Homo sapiens |