Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Rattus norvegicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0032 | - |
thioredoxin disulfide | purified, recombinant, tetrameric enzyme | Rattus norvegicus | |
0.0036 | - |
thioredoxin disulfide | purified, recombinant, dimeric enzyme | Rattus norvegicus | |
0.139 | - |
5,5'-dithiobis(2-nitrobenzoic acid) | purified, recombinant, tetrameric enzyme | Rattus norvegicus | |
0.2156 | - |
5,5'-dithiobis(2-nitrobenzoic acid) | purified, recombinant, dimeric enzyme | Rattus norvegicus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
selenium | TrxR1 is a selenoprotein that contains a selenocysteine residue at the penultimate C-terminal | Rattus norvegicus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
54386 | - |
2 * 54386, highly active enzyme form, calculated from amino acid sequence | Rattus norvegicus |
150000 | - |
highly active enzyme form, gel filtration | Rattus norvegicus |
250000 | - |
low-activity enzyme form, gel filtration | Rattus norvegicus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | O89049 | - |
- |
Purification (Comment) | Organism |
---|---|
2',5'-ADP Sepharose column chromatography and phenylarsine oxide Sepharose column chromatography | Rattus norvegicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
5,5'-dithiobis(2-nitrobenzoic acid) + NADPH + H+ | - |
Rattus norvegicus | 2-nitro-5-thiobenzoate + NADP+ | - |
? | |
thioredoxin disulfide + NADPH + H+ | - |
Rattus norvegicus | thioredoxin + NADP+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | 2 * 54386, highly active enzyme form, calculated from amino acid sequence | Rattus norvegicus |
Synonyms | Comment | Organism |
---|---|---|
thioredoxin reductase 1 | - |
Rattus norvegicus |
TrxR1 | - |
Rattus norvegicus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
33.08 | - |
5,5'-dithiobis(2-nitrobenzoic acid) | purified, recombinant, tetrameric enzyme | Rattus norvegicus | |
47 | 73 | thioredoxin disulfide | purified, recombinant, tetrameric enzyme | Rattus norvegicus | |
106.3 | - |
5,5'-dithiobis(2-nitrobenzoic acid) | purified, recombinant, dimeric enzyme | Rattus norvegicus | |
114.1 | - |
thioredoxin disulfide | purified, recombinant, dimeric enzyme | Rattus norvegicus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | - |
Rattus norvegicus | |
NADPH | - |
Rattus norvegicus |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
183.3 | - |
5,5'-dithiobis(2-nitrobenzoic acid) | purified, recombinant, tetrameric enzyme | Rattus norvegicus | |
495 | - |
5,5'-dithiobis(2-nitrobenzoic acid) | purified, recombinant, dimeric enzyme | Rattus norvegicus | |
14920 | - |
thioredoxin disulfide | purified, recombinant, tetrameric enzyme | Rattus norvegicus | |
31700 | - |
thioredoxin disulfide | purified, recombinant, dimeric enzyme | Rattus norvegicus |