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Literature summary for 1.8.1.9 extracted from

  • Du, Y.; Wu, Y.; Cao, X.; Cui, W.; Zhang, H.; Tian, W.; Ji, M.; Holmgren, A.; Zhong, L.
    Inhibition of mammalian thioredoxin reductase by black tea and its constituents: implications for anticancer actions (2009), Biochimie, 91, 434-444.
    View publication on PubMed
Search for more literature for 1.8.1.9

Inhibitors

Inhibitors Comment Organism Structure
1-methyl-1-propyl-2-imidazolyl disulfide
-
 Bos taurus
1-methyl-1-propyl-2-imidazolyl disulfide
-
 Homo sapiens
curcumin
-
 Bos taurus
curcumin
-
 Homo sapiens
Hg2+
-
 Bos taurus
Hg2+
-
 Homo sapiens
additional information black tea extract and theaflavins (mixture of theaflavin, theaflavin-3-monogallate, theaflavin-3'-monogallate and theaflavin-3,3'-digallate) inhibit the purified TrxR1 with IC50 44 mg/ml and 21 mg/ml, respectively. Kinetics of theaflavins exhibit a mixed type of competitive and non-competitive inhibition, with Kis 4 mg/ml and Kii 26 mg/ml against coenzyme NADPH, and with Kis 12 mg/ml and Kii 27 mg/ml against substrate 5,5'-dithiobis(2-nitrobenzoic acid) Homo sapiens
theaflavin
-
 Bos taurus
theaflavin-3'-monogallate
-
 Bos taurus
theaflavin-3,3'-digallate
-
 Bos taurus
theaflavin-3-monogallate
-
 Bos taurus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
55000
-
 2 * 55000, SDS-PAGE Homo sapiens
110000
-
 gel filtration Homo sapiens

Organism

Organism UniProt Comment Textmining
Bos taurus O62768
-
-
Homo sapiens Q16881
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
EAhy 926 cell
-
 Homo sapiens
-
HeLa cell
-
 Homo sapiens
-
liver
-
 Bos taurus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5,5'-dithiobis(2-nitrobenzoic acid) + NADPH + H+
-
 Bos taurus 2-nitro-5-thiobenzoate + NADP+
-
 ?

Subunits

Subunits Comment Organism
homodimer 2 * 55000, SDS-PAGE Homo sapiens

Synonyms

Synonyms Comment Organism
thioredoxin reductase
-
 Homo sapiens
thioredoxin reductase
-
 Bos taurus
TrxR1
-
 Homo sapiens
TrxR1
-
 Bos taurus

Cofactor

Cofactor Comment Organism Structure
NADPH
-
 Homo sapiens
NADPH
-
 Bos taurus

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
additional information
-
 additional information kinetics of theaflavins exhibit a mixed type of competitive and non-competitive inhibition, with Kis 4 mg/ml and Kii 26 mg/ml against coenzyme NADPH, and with Kis 12 mg/ml and Kii 27 mg/ml against substrate 5,5'-dithiobis(2-nitrobenzoic acid) Homo sapiens

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
additional information
-
 black tea extract and theaflavins (mixture of theaflavin, theaflavin-3-monogallate, theaflavin-3'-monogallate and theaflavin-3,3'-digallate) inhibit the purified TrxR1 with IC50 44 mg/ml and 21 mg/ml, respectively Homo sapiens additional information
0.018
-
 in 50 mM potassium phosphate, 1 mM EDTA, pH 7.5 Bos taurus theaflavin-3-monogallate
0.0191
-
 in 50 mM potassium phosphate, 1 mM EDTA, pH 7.5 Bos taurus theaflavin-3'-monogallate
0.0216
-
 in 50 mM potassium phosphate, 1 mM EDTA, pH 7.5 Bos taurus theaflavin-3,3'-digallate
0.06
-
 IC50 above 0.06 mM, in 50 mM potassium phosphate, 1 mM EDTA, pH 7.5 Bos taurus theaflavin