Literature summary for 1.8.1.9 extracted from

Gray, J.P.; Heck, D.E.; Mishin, V.; Smith, P.J.; Hong, J.Y.; Thiruchelvam, M.; Cory-Slechta, D.A.; Laskin, D.L.; Laskin, J.D.
Paraquat increases cyanide-insensitive respiration in murine lung epithelial cells by activating an NAD(P)H: paraquat oxidoreductase: Identification of the enzyme as thioredoxin reductase (2007), J. Biol. Chem., 282, 7939-7949.

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Inhibitors

Inhibitors	Comment	Organism	Structure
1-chloro-2,4-dinitrobenzene	0.1 mM	Mus musculus
diphenylene iodonium	IC50: 0.001 mM	Mus musculus
sodium aurothiomalate	0.1 mM	Mus musculus

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
additional information	-	additional information	effects of paraquat on reaction kinetics of NADH and NADPH in lung cell extracts	Mus musculus

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
cytoplasm	-	Mus musculus	5737	-

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
58000	-	SDS-PAGE	Mus musculus

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
additional information	Mus musculus	the enzyme can promote oxidative stress by redox cycling of paraquat: paraquat + O2 + NADPH + H+ --> paraquat radical + O2- radical + NADP+	?	-	?

Organism

Organism	UniProt	Comment	Textmining
Mus musculus	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
-	Mus musculus
2',5'-ADP-agarose affinity chromatography and Superose size exclusion chromatography	Mus musculus

Source Tissue

Source Tissue	Comment	Organism	Textmining
lung epithelium	-	Mus musculus	-
lung epithelium	MLE-15 cell line	Mus musculus	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
5,5'-dithiobis(2-nitrobenzoic acid) + NADPH	-	Mus musculus	2-nitro-5-thiobenzoate + NADP+	-	?
5,5'-dithiobis(2-nitrobenzoic acid) + NADPH + H+	-	Mus musculus	2-nitro-5-thiobenzoate + NADP+	-	?
additional information	the enzyme can promote oxidative stress by redox cycling of paraquat: paraquat + O2 + NADPH + H+ --> paraquat radical + O2- radical + NADP+	Mus musculus	?	-	?
paraquat + NAD(P)H	-	Mus musculus	paraquat radical + NAD(P)+	-	r
paraquat radical + O2	-	Mus musculus	paraquat + O2-	-	r

Synonyms

Synonyms	Comment	Organism
NAD(P)H:paraquat oxidoreductase	-	Mus musculus
thioredoxin reductase	-	Mus musculus
TrxR	-	Mus musculus

Cofactor

Cofactor	Comment	Organism	Structure
FAD	-	Mus musculus
NADH	-	Mus musculus
NADPH	-	Mus musculus
NADPH	approximately four times more activity was evident with NADPH when compared to NADH	Mus musculus

IC50 Value

IC50 Value	IC50 Value Maximum	Comment	Organism	Inhibitor	Structure
0.001	-	IC50: 0.001 mM	Mus musculus	diphenylene iodonium

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Release 2023.1 (January 2023)