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Literature summary for 1.8.1.9 extracted from
- Characterization of alternative cytosolic forms and cellular targets of mouse mitochondrial thioredoxin reductase (2006), J. Biol. Chem., 281, 22953-22963.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| EDTA | if the concentration of EDTA in the reaction mixture is higher than 0.1 mM the enzyme shows full activity | Mus musculus |  |
| EDTA | if the concentration of EDTA in the reaction mixture is higher than 0.1 mM the enzyme shows full activity | Homo sapiens | |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli BL21(DE3) cells | Mus musculus |
| expressed in Escherichia coli BL21(DE3) cells | Homo sapiens |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| additional information | no inhibition by Ca2+ or Co2+ | Homo sapiens | |
| additional information | no inhibition by Ca2+ or Co2+ | Mus musculus | |
| Zn2+ | 0.1-0.2 mM and above | Homo sapiens | |
| Zn2+ | 0.1-0.2 mM and above | Mus musculus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.00141 | - |
thioredoxin | rat thioredoxin as substrate | Homo sapiens | |
| 0.00157 | - |
thioredoxin | Escherichia coli thioredoxin as substrate | Homo sapiens | |
| 0.00183 | - |
thioredoxin | human thioredoxin as substrate | Homo sapiens | |
| 0.00236 | - |
thioredoxin | human thioredoxin as substrate | Mus musculus | |
| 0.00256 | - |
thioredoxin | Escherichia coli thioredoxin as substrate | Mus musculus | |
| 0.0051 | - |
thioredoxin | rat thioredoxin as substrate | Mus musculus | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytosol | - |
Homo sapiens | 5829 | - |
| cytosol | TR1 | Mus musculus | 5829 | - |
| mitochondrion | TR3 | Mus musculus | 5739 | - |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
- |
- |
| Mus musculus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| Sepharose affinity column chromatography | Homo sapiens |
| TALONTM column chromatography and Sepharose affinity column chromatography | Mus musculus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| liver | - |
Mus musculus | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | TR3 does not have catalytic preferences for mitochondrial thioredoxin versus cytosolic thioredoxin | Mus musculus | ? | - |
? | |
| thioredoxin + NADP+ | - |
Mus musculus | thioredoxin disulfide + NADPH | - |
? | |
| thioredoxin + NADP+ | - |
Homo sapiens | thioredoxin disulfide + NADPH | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| thioredoxin reductase | - |
Mus musculus |
| thioredoxin reductase | - |
Homo sapiens |
| TR1 | - |
Homo sapiens |
| TR3 | - |
Mus musculus |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.016 | - |
thioredoxin | Escherichia coli thioredoxin as substrate | Homo sapiens | |
| 3.5 | - |
thioredoxin | human thioredoxin as substrate | Mus musculus | |
| 8.1 | - |
thioredoxin | human thioredoxin as substrate | Homo sapiens | |
| 19.97 | - |
thioredoxin | human thioredoxin as substrate | Mus musculus | |
| 25.78 | - |
thioredoxin | human thioredoxin as substrate | Homo sapiens | |
| 27.4 | - |
thioredoxin | rat thioredoxin as substrate | Homo sapiens | |
| 29.5 | - |
thioredoxin | Escherichia coli thioredoxin as substrate | Mus musculus | |
| 37.88 | - |
thioredoxin | rat thioredoxin as substrate | Mus musculus | |
| 46.57 | - |
thioredoxin | Escherichia coli thioredoxin as substrate | Homo sapiens | |
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