Activating Compound | Comment | Organism | Structure |
---|---|---|---|
EDTA | if the concentration of EDTA in the reaction mixture is higher than 0.1 mM the enzyme shows full activity | Mus musculus | |
EDTA | if the concentration of EDTA in the reaction mixture is higher than 0.1 mM the enzyme shows full activity | Homo sapiens |
Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Mus musculus |
expressed in Escherichia coli BL21(DE3) cells | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | no inhibition by Ca2+ or Co2+ | Homo sapiens | |
additional information | no inhibition by Ca2+ or Co2+ | Mus musculus | |
Zn2+ | 0.1-0.2 mM and above | Homo sapiens | |
Zn2+ | 0.1-0.2 mM and above | Mus musculus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00141 | - |
thioredoxin | rat thioredoxin as substrate | Homo sapiens | |
0.00157 | - |
thioredoxin | Escherichia coli thioredoxin as substrate | Homo sapiens | |
0.00183 | - |
thioredoxin | human thioredoxin as substrate | Homo sapiens | |
0.00236 | - |
thioredoxin | human thioredoxin as substrate | Mus musculus | |
0.00256 | - |
thioredoxin | Escherichia coli thioredoxin as substrate | Mus musculus | |
0.0051 | - |
thioredoxin | rat thioredoxin as substrate | Mus musculus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Homo sapiens | 5829 | - |
cytosol | TR1 | Mus musculus | 5829 | - |
mitochondrion | TR3 | Mus musculus | 5739 | - |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Mus musculus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Sepharose affinity column chromatography | Homo sapiens |
TALONTM column chromatography and Sepharose affinity column chromatography | Mus musculus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Mus musculus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | TR3 does not have catalytic preferences for mitochondrial thioredoxin versus cytosolic thioredoxin | Mus musculus | ? | - |
? | |
thioredoxin + NADP+ | - |
Mus musculus | thioredoxin disulfide + NADPH | - |
? | |
thioredoxin + NADP+ | - |
Homo sapiens | thioredoxin disulfide + NADPH | - |
? |
Synonyms | Comment | Organism |
---|---|---|
thioredoxin reductase | - |
Mus musculus |
thioredoxin reductase | - |
Homo sapiens |
TR1 | - |
Homo sapiens |
TR3 | - |
Mus musculus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.016 | - |
thioredoxin | Escherichia coli thioredoxin as substrate | Homo sapiens | |
3.5 | - |
thioredoxin | human thioredoxin as substrate | Mus musculus | |
8.1 | - |
thioredoxin | human thioredoxin as substrate | Homo sapiens | |
19.97 | - |
thioredoxin | human thioredoxin as substrate | Mus musculus | |
25.78 | - |
thioredoxin | human thioredoxin as substrate | Homo sapiens | |
27.4 | - |
thioredoxin | rat thioredoxin as substrate | Homo sapiens | |
29.5 | - |
thioredoxin | Escherichia coli thioredoxin as substrate | Mus musculus | |
37.88 | - |
thioredoxin | rat thioredoxin as substrate | Mus musculus | |
46.57 | - |
thioredoxin | Escherichia coli thioredoxin as substrate | Homo sapiens |