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Literature summary for 1.8.1.9 extracted from
- Characterization of mitochondrial thioredoxin reductase from C. elegans (2006), Biochem. Biophys. Res. Commun., 346, 629-636.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli | Caenorhabditis elegans |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| TR-GCCS | mutant with C-terminal sequence of GCCS | Caenorhabditis elegans |
| TR-SCCS | mutant with C-terminal sequence of SCCS | Caenorhabditis elegans |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.077 | - |
thioredoxin | thioredoxin from Escherichia coli, mutant TR-GCCS | Caenorhabditis elegans |  |
| 0.219 | - |
thioredoxin | thioredoxin from Escherichia coli, mutant TR-SCCS | Caenorhabditis elegans | |
| 0.42 | - |
5,5'-dithiobis(2-nitrobenzoic acid) | wild type enzyme | Caenorhabditis elegans | |
| 0.61 | - |
thioredoxin | thioredoxin from Escherichia coli, wild type enzyme | Caenorhabditis elegans | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| mitochondrion | - |
Caenorhabditis elegans | 5739 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 55000 | - |
SDS-PAGE | Caenorhabditis elegans |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Caenorhabditis elegans | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| chitin-agarose column gel filtration and S200 column size-exclusion chromatography | Caenorhabditis elegans |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 5,5'-dithiobis(2-nitrobenzoic acid) + NADPH | - |
Caenorhabditis elegans | 2-nitro-5-thiobenzoate + NADP+ | - |
? | |
| additional information | the enzyme does not reduce hydrogen peroxide or insulin | Caenorhabditis elegans | ? | - |
? | |
| selenocysteine + NADPH | - |
Caenorhabditis elegans | selenocystine + NADP+ | - |
? | |
| thioredoxin + NADP+ | - |
Caenorhabditis elegans | thioredoxin disulfide + NADPH | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| CeTR2 | mitochondrial TR from Caenorhabditis elegans | Caenorhabditis elegans |
| thioredoxin reductase | - |
Caenorhabditis elegans |
| TR | - |
Caenorhabditis elegans |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.052 | 2.1 | thioredoxin | thioredoxin from Escherichia coli, wild type enzyme | Caenorhabditis elegans | |
| 2.23 | - |
5,5'-dithiobis(2-nitrobenzoic acid) | wild type enzyme | Caenorhabditis elegans | |
| 4.03 | - |
thioredoxin | thioredoxin from Escherichia coli, mutant TR-SCCS | Caenorhabditis elegans | |
| 5.3 | - |
thioredoxin | thioredoxin from Escherichia coli, mutant TR-GCCS | Caenorhabditis elegans | |
| 10.17 | - |
thioredoxin | thioredoxin from Escherichia coli, wild type enzyme | Caenorhabditis elegans | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
- |
Caenorhabditis elegans |
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