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Literature summary for 1.8.1.9 extracted from

  • Arner, E.S.J.; Bjoernstedt, M.; Holmgren, A.
    1-Chloro-2,4-dinitrobenzene is an irreversible inhibitor of human thioredoxin reductase. Loss of thioredoxin disulfide reductase activity is accompanied by a large increase in NADPH oxidase activity (1995), J. Biol. Chem., 270, 3479-3482.
    View publication on PubMed
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Activating Compound

Activating Compound Comment Organism Structure
cysteine part of the active site Homo sapiens

Application

Application Comment Organism
medicine target for immunostimulating effect of 1-chloro-2,4-dinitrobenzene as agent in AIDS treatment Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
1-chloro-2,4-dinitrobenzene i.e. DNCB; irreversible, with NADPH, alkylation of the active site cysteine disulfide, strong increase in oxidation activity of the enzyme against NADPH Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Reaction

Reaction Comment Organism Reaction ID
thioredoxin + NADP+ = thioredoxin disulfide + NADPH + H+ inhibition mechanism Homo sapiens
a
thioredoxin + NADP+ = thioredoxin disulfide + NADPH + H+ electron transfer in the enzyme complex of apoenzyme, FAD and thioredoxin with NADPH Homo sapiens
a

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
thioredoxin + NADP+ coupled assay with DTNB Homo sapiens thioredoxin disulfide + NADPH
-
 r
thioredoxin + NADP+
-
 Homo sapiens thioredoxin disulfide + NADPH + H+
-
 r

Synonyms

Synonyms Comment Organism
TR
-
 Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
20
-
 assay at Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
 assay at Homo sapiens

Cofactor

Cofactor Comment Organism Structure
FAD
-
 Homo sapiens
NADPH
-
 Homo sapiens