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Literature summary for 1.8.1.9 extracted from

  • Krnajski, Z.; Gilberger, T.W.; Walter, R.D.; Muller, S.
    Intersubunit interactions in Plasmodium falciparum thioredoxin reductase (2000), J. Biol. Chem., 275, 40874-40878.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
cysteine 1 pair of redox active cysteines at the N-terminal active center and 1 pair of selenenylsulfide at the C-terminus, crucial for the reduction of thioredoxin Plasmodium falciparum

Application

Application Comment Organism
medicine target for a chemotherapeutic intervention of malaria Plasmodium falciparum

Cloned(Commentary)

Cloned (Comment) Organism
expression of His-tagged or Strep-tagged wild-type and mutants in Escherichia coli, DNA sequence analysis Plasmodium falciparum

Protein Variants

Protein Variants Comment Organism
C535S construction of a homodimer and heterodimer, the latter containing 1 mutant and 1 wild-type subunit, activity is reduced by 56 and 92%, respectively Plasmodium falciparum
C535S/C88A double mutant, construction of a homodimer and a heterodimer, the latter containing 1 mutant and 1 wild-type subunit, activity is reduced by 89 and 95%, respectively Plasmodium falciparum
C88A construction of a homodimer and a heterodimer, the latter containing 1 mutant and 1 wild-type subunit, activity is reduced by 4 and 90% Plasmodium falciparum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
thioredoxin + NADP+ Plasmodium falciparum detoxification of hydrogen peroxide, protection of the cell against oxidative damage thioredoxin disulfide + NADPH
-
?

Organism

Organism UniProt Comment Textmining
Plasmodium falciparum
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged wild-type and mutant enzymes from Escherichia coli Plasmodium falciparum

Reaction

Reaction Comment Organism Reaction ID
thioredoxin + NADP+ = thioredoxin disulfide + NADPH + H+ catalytic mechanism Plasmodium falciparum
thioredoxin + NADP+ = thioredoxin disulfide + NADPH + H+ oxidation-reduction cycle of thioredoxin Plasmodium falciparum

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
quantification by DTNB coupled assay method Plasmodium falciparum
0.2
-
purified double mutant C535S/C88A as homodimer, insulin coupled assay Plasmodium falciparum
0.3
-
purified mutant C535S as homodimer, insulin coupled assay Plasmodium falciparum
0.4
-
homodimer of mutant C88A, insulin coupled assay Plasmodium falciparum
0.4
-
purified double mutant C535S/C88A as heterodimer with the wild-type, insulin coupled assay Plasmodium falciparum
1.5
-
purified mutant C535A as heterodimer with the wild-type, insulin coupled assay Plasmodium falciparum
2.2
-
purified mutant C88A as heterodimer with the wild-type, insulin coupled assay Plasmodium falciparum
3.9
-
purified wild-type enzyme, insulin coupled assay Plasmodium falciparum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5,5'-dithiobis(2-nitrobenzoic acid) + NADPH i.e. DTNB Plasmodium falciparum 2-nitro-5-thiobenzoate + NADP+
-
?
5,5'-dithiobis(2-nitrobenzoic acid) + NADPH coupled assay Plasmodium falciparum 2-nitro-5-thiobenzoate + NADP+
-
?
additional information redox system for electron transfer in the complex of apoenzyme, FAD and thioredoxin with NADPH or other electron acceptors Plasmodium falciparum ?
-
?
thioredoxin + NADP+ coupled assay with DTNB Plasmodium falciparum thioredoxin disulfide + NADPH
-
?
thioredoxin + NADP+ coupled assay, measurement of NADPH oxidation in presence of insulin and thioredoxin Plasmodium falciparum thioredoxin disulfide + NADPH
-
?
thioredoxin + NADP+ detoxification of hydrogen peroxide, protection of the cell against oxidative damage Plasmodium falciparum thioredoxin disulfide + NADPH
-
?
thioredoxin + NADP+ in presence of NADPH, coupled assay Plasmodium falciparum thioredoxin disulfide + NADPH + H+
-
?

Subunits

Subunits Comment Organism
dimer
-
Plasmodium falciparum
More intersubunit organisation Plasmodium falciparum

Synonyms

Synonyms Comment Organism
More 2 classes of thioredoxin reductases: high molecular weight or large proteins e.g. in mammalia, Plasmodium falsiparum and Caenorhabditis elegans, and low molecular weight proteins e.g. in bacteria Plasmodium falciparum
TrxT
-
Plasmodium falciparum

Cofactor

Cofactor Comment Organism Structure
FAD
-
Plasmodium falciparum
NADPH
-
Plasmodium falciparum