Activating Compound | Comment | Organism | Structure |
---|---|---|---|
cysteine | part of the active site | Arabidopsis thaliana |
Cloned (Comment) | Organism |
---|---|
strong overexpression in Escherichia coli, DNA sequence analysis | Arabidopsis thaliana |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | additionally to the Arabidopsis thaliana thioredoxin, several other thioredoxins have been tested, Km-values | Arabidopsis thaliana | |
0.0011 | - |
thioredoxin | recombinant enzyme | Arabidopsis thaliana |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Arabidopsis thaliana | 5829 | - |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | - |
EMBL accession numbers Z23109, Z23108 | - |
Purification (Comment) | Organism |
---|---|
recombinant from Escherichia coli | Arabidopsis thaliana |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
thioredoxin + NADP+ | - |
Arabidopsis thaliana | thioredoxin disulfide + NADPH | - |
? | |
thioredoxin + NADP+ | i.e. DTNB | Arabidopsis thaliana | thioredoxin disulfide + NADPH + H+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 35000-35328, SDS-PAGE and amino acid analysis | Arabidopsis thaliana |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
5 min, active | Arabidopsis thaliana |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | - |
Arabidopsis thaliana | |
NADPH | specific for | Arabidopsis thaliana |