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Literature summary for 1.8.1.7 extracted from

  • Karaman, M.; Akkemik, E.; Budak, H.; Ciftci, M.
    In vitro effects of some drugs on human erythrocyte glutathione reductase (2012), J. Enzyme Inhib. Med. Chem., 27, 18-23.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
gadopentetic acid non-competitive inhibition Homo sapiens
ketotifen non-competitive inhibition Homo sapiens
meloxicam non-competitive inhibition Homo sapiens
phenyramidol competitive inhibition Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
glutathione disulfide + NADPH + H+ Homo sapiens
-
glutathione + NADP+
-
?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate precipitation, 2‘‘,5‘-ADP Sepharose 4B affinity column chromatography, and Sephadex G-200 gel filtration Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
erythrocyte
-
Homo sapiens
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.5
-
enzyme from hemolysate, at pH 8.0 and 25°C Homo sapiens
314
-
after 209fold purification, at pH 8.0 and 25°C Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
glutathione disulfide + NADPH + H+
-
Homo sapiens glutathione + NADP+
-
?

Synonyms

Synonyms Comment Organism
glutathione:NADP+ oxidoreductase
-
Homo sapiens

Cofactor

Cofactor Comment Organism Structure
NADPH
-
Homo sapiens

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.0008
-
ketotifen in 40 mM Tris-HCl buffer, pH 8.0, at 25°C Homo sapiens
0.0061
-
meloxicam in 40 mM Tris-HCl buffer, pH 8.0, at 25°C Homo sapiens
0.434
-
phenyramidol in 40 mM Tris-HCl buffer, pH 8.0, at 25°C Homo sapiens
28.84
-
gadopentetic acid in 40 mM Tris-HCl buffer, pH 8.0, at 25°C Homo sapiens

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.012
-
in 40 mM Tris-HCl buffer, pH 8.0, at 25°C Homo sapiens ketotifen
0.029
-
in 40 mM Tris-HCl buffer, pH 8.0, at 25°C Homo sapiens meloxicam
0.99
-
in 40 mM Tris-HCl buffer, pH 8.0, at 25°C Homo sapiens phenyramidol
138
-
in 40 mM Tris-HCl buffer, pH 8.0, at 25°C Homo sapiens gadopentetic acid