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Literature summary for 1.8.1.7 extracted from

  • Can, B.; Kulaksiz Erkmen, G.; Dalmizrak, O.; Ogus, I.H.; Ozer, N.
    Purification and characterisation of rat kidney glutathione reductase (2010), Protein J., 29, 250-256.
    View publication on PubMed

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0153
-
NADPH in 100 mM potassium phosphate buffer, pH 7.4, 4 mM EDTA, at 37°C Rattus norvegicus
0.0531
-
glutathione disulfide in 100 mM potassium phosphate buffer, pH 7.4, 4 mM EDTA, at 37°C Rattus norvegicus

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
Rattus norvegicus 5829
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
53000
-
native PAGE and SDS-PAGE Rattus norvegicus

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
heat-denaturation and Sephadex G25 gel filtration, 2',5'-ADP agarose 4B column chromatography, and PBE94 column chromatography Rattus norvegicus

Source Tissue

Source Tissue Comment Organism Textmining
kidney
-
Rattus norvegicus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.11
-
crude liver homogenate, in 100 mM potassium phosphate buffer, pH 7.4, 4 mM EDTA, at 37°C Rattus norvegicus
250
-
after 2356fold purification, in 100 mM potassium phosphate buffer, pH 7.4, 4 mM EDTA, at 37°C Rattus norvegicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
glutathione disulfide + NADH + H+ NADH is used as a coenzyme with a lower efficiency (32.7%) than NADPH Rattus norvegicus glutathione + NAD+
-
?
glutathione disulfide + NADPH + H+
-
Rattus norvegicus glutathione + NADP+
-
?

Synonyms

Synonyms Comment Organism
glutathione reductase
-
Rattus norvegicus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
65
-
-
Rattus norvegicus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5
-
-
Rattus norvegicus

Cofactor

Cofactor Comment Organism Structure
FAD each mol of enzyme subunit binds 0.97 mol of FAD Rattus norvegicus
NADH NADH is used as a coenzyme by rat kidney glutathione reductase but with a lower efficiency (32.7%) than NADPH Rattus norvegicus
NADPH
-
Rattus norvegicus

General Information

General Information Comment Organism
physiological function glutathione reductase is the crucial enzyme to maintain high glutathione/glutathione disulfide ratio and physiological redox status in cells Rattus norvegicus

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
4850
-
glutathione disulfide in 100 mM potassium phosphate buffer, pH 7.4, 4 mM EDTA, at 37°C Rattus norvegicus
16800
-
NADPH in 100 mM potassium phosphate buffer, pH 7.4, 4 mM EDTA, at 37°C Rattus norvegicus