Activating Compound | Comment | Organism | Structure |
---|---|---|---|
FAD analogues | - |
Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Melarsen oxide | 2-step process | Homo sapiens | |
Melarsen oxide | i.e. p-(4,6-diamono-s-triazin-2-yl)aminophenylarsenoxide; potent inhibitor, reversible by excess of thiols, more sensitive with NADPH | Trypanosoma brucei gambiense | |
Melarsen oxide | i.e. p-(4,6-diamono-s-triazin-2-yl)aminophenylarsenoxide; potent inhibitor, reversible by excess of thiols, more sensitive with NADPH | Trypanosoma brucei rhodesiense | |
NADPH | protection by NADP+ | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bos taurus | - |
- |
- |
Homo sapiens | - |
- |
- |
Trypanosoma brucei gambiense | - |
parasitic protozoa | - |
Trypanosoma brucei rhodesiense | - |
parasitic protozoa | - |
Purification (Comment) | Organism |
---|---|
- |
Homo sapiens |
- |
Bos taurus |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
2 glutathione + NADP+ = glutathione disulfide + NADPH + H+ | inhibition mechanism | Homo sapiens | |
2 glutathione + NADP+ = glutathione disulfide + NADPH + H+ | analogous to trypanothione reductase | Trypanosoma brucei rhodesiense | |
2 glutathione + NADP+ = glutathione disulfide + NADPH + H+ | analogous to trypanothione reductase | Trypanosoma brucei gambiense |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
erythrocyte | - |
Homo sapiens | - |
intestine | mucosa | Bos taurus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
114 | - |
purified enzyme | Bos taurus |
262 | - |
purified enzyme | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
GSSG + NADPH | - |
Homo sapiens | glutathione + NADP+ | - |
? | |
GSSG + NADPH | - |
Bos taurus | glutathione + NADP+ | - |
? | |
GSSG + NADPH | - |
Trypanosoma brucei rhodesiense | glutathione + NADP+ | - |
? | |
GSSG + NADPH | - |
Trypanosoma brucei gambiense | glutathione + NADP+ | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
27 | - |
assay at | Homo sapiens |
27 | - |
assay at | Bos taurus |
27 | - |
assay at | Trypanosoma brucei rhodesiense |
27 | - |
assay at | Trypanosoma brucei gambiense |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.8 | - |
assay at | Homo sapiens |
7.8 | - |
assay at | Bos taurus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | FAD enzyme | Homo sapiens | |
FAD | FAD enzyme | Bos taurus | |
FAD | FAD enzyme | Trypanosoma brucei rhodesiense | |
FAD | FAD enzyme | Trypanosoma brucei gambiense |