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Literature summary for 1.8.1.7 extracted from
- The purification and properties of glutathione reductase from the cestode Moniezia expansa (1995), Int. J. Biochem. Cell Biol., 27, 393-401.
Application
| Application | Comment | Organism |
|---|---|---|
| medicine | potential target for chemotherapy | Moniezia expansa |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 1,3-Bis-(2-chloroethyl)-1-nitrosourea | - |
Moniezia expansa |  |
| 2,4,6-Trinitrobenzenesulfonate | - |
Moniezia expansa | |
| 5-nitro-2-furoic acid | - |
Moniezia expansa | |
| 5-nitroindole | - |
Moniezia expansa | |
| GSSG | - |
Moniezia expansa | |
| Na+ | - |
Moniezia expansa | |
| Nitrofurantoin | - |
Moniezia expansa | |
| nitrofurazone | - |
Moniezia expansa | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.021 | - |
NADPH | - |
Moniezia expansa | |
| 0.076 | - |
GSSG | - |
Moniezia expansa | |
| 0.35 | - |
NADH | - |
Moniezia expansa | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytoplasm | - |
Moniezia expansa | 5737 | - |
| mitochondrion | small amount | Moniezia expansa | 5739 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 63000 | - |
2 * 63000, SDS-PAGE | Moniezia expansa |
| 114000 | - |
- |
Moniezia expansa |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| GSSG + NADPH | Moniezia expansa | maintenance of glutathione/GSSG ratio is a protective mechanism for intracellular thiols during growth in atmospheric oxygen | glutathione + NADP+ | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Moniezia expansa | - |
cestode | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Moniezia expansa |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 5.03 | - |
purified enzyme | Moniezia expansa |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 4°C, 50 mM potassium phosphate, pH 7.4, 50% loss of activity after 10 days | Moniezia expansa |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| glutathione disulfide + NADH + H+ | - |
Moniezia expansa | glutathione + NAD+ | - |
? | |
| GSSG + NADPH | mixed disulfide between coenzyme A and glutathione | Moniezia expansa | glutathione + NADP+ | - |
? | |
| GSSG + NADPH | maintenance of glutathione/GSSG ratio is a protective mechanism for intracellular thiols during growth in atmospheric oxygen | Moniezia expansa | glutathione + NADP+ | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 63000, SDS-PAGE | Moniezia expansa |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Moniezia expansa |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5 | - |
NADH + GSSG | Moniezia expansa |
| 7.4 | - |
NADPH + GSSG | Moniezia expansa |
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Release 2023.1 (January 2023)
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