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Literature summary for 1.8.1.7 extracted from
- Glutathione reductase (yeast) (1971), Methods Enzymol., 17, 500-503.
No PubMed abstract available
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| iodoacetate | in presence but not in absence of reduced coenzyme | Saccharomyces cerevisiae |  |
| sulfhydryl reagents | in presence but not in absence of reduced coenzyme | Saccharomyces cerevisiae | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0038 | - |
NADPH | - |
Saccharomyces cerevisiae | |
| 0.055 | - |
GSSG | - |
Saccharomyces cerevisiae | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 118000 | - |
equilibrium sedimentation | Saccharomyces cerevisiae |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Saccharomyces cerevisiae | - |
baker's yeast | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Saccharomyces cerevisiae |
Renatured (Commentary)
| Renatured (Comment) | Organism |
|---|---|
| refolding after treatment with 6 M urea, recovering of full activity | Saccharomyces cerevisiae |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 153 | - |
- |
Saccharomyces cerevisiae |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, pH 6.8, 0.05 M potassium phosphate buffer, indefinitely stable | Saccharomyces cerevisiae |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| GSSG + NADPH | - |
Saccharomyces cerevisiae | glutathione + NADP+ | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
assay at | Saccharomyces cerevisiae |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.6 | 7.6 | - |
Saccharomyces cerevisiae |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 3 | - |
stable | Saccharomyces cerevisiae |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| FAD | FAD enzyme | Saccharomyces cerevisiae | |
| NADPH | - |
Saccharomyces cerevisiae | |
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Release 2023.1 (January 2023)
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