Protein Variants | Comment | Organism |
---|---|---|
C82A | mutagenesis of the interchange thiol, abolishes all redox-dependent reactions. Redox-independent acetoacetate decarboxylation is not decreased | Xanthobacter autotrophicus |
C87A | mutagenesis of the flavin thiol, results in an inactive enzyme for steady-state redox-dependent reactions, but this variant catalyzes a single-turnover reaction producing a 0.8:1 ratio of product to enzyme. Redox-independent acetoacetate decarboxylation is not decreased | Xanthobacter autotrophicus |
H137A | mutagenesis of the histidine proximal to the ordered water molecule, leads to nearly complete loss of redox-dependent reactions. Redox-independent acetoacetate decarboxylation is not decreased | Xanthobacter autotrophicus |
H84A | mutagenesis of the distal histidine residue, reduces the redox-dependent activities by 58 to 76%. Redox-independent acetoacetate decarboxylation is not decreased | Xanthobacter autotrophicus |
M140A | residue flanking the substrate, catalytic efficiency for 2-(2-oxopropylthio)ethanesulfonate carboxylation is 47fold lower than that for wild-type | Xanthobacter autotrophicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.42 | - |
2-(2-oxopropylthio)ethanesulfonate | wild-type, pH 7.4, 30°C | Xanthobacter autotrophicus | |
6.37 | - |
2-(2-oxopropylthio)ethanesulfonate | mutant M140A, pH 7.4, 30°C | Xanthobacter autotrophicus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Xanthobacter autotrophicus | Q56839 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-(2-oxopropylthio)ethanesulfonate + CO2 + NADPH + H+ | - |
Xanthobacter autotrophicus | 2-mercaptoethanesulfonate + acetoacetate + NADP+ | - |
? |
Synonyms | Comment | Organism |
---|---|---|
xecC | - |
Xanthobacter autotrophicus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.072 | - |
2-(2-oxopropylthio)ethanesulfonate | mutant M140A, pH 7.4, 30°C | Xanthobacter autotrophicus | |
0.22 | - |
2-(2-oxopropylthio)ethanesulfonate | wild-type, pH 7.4, 30°C | Xanthobacter autotrophicus |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.011 | - |
2-(2-oxopropylthio)ethanesulfonate | mutant M140A, pH 7.4, 30°C | Xanthobacter autotrophicus | |
0.53 | - |
2-(2-oxopropylthio)ethanesulfonate | wild-type, pH 7.4, 30°C | Xanthobacter autotrophicus |