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Literature summary for 1.8.1.4 extracted from

  • Jolley, K.A.; Rapaport, E.; Hough, D.W.; Danson, M.J.; Woods, W.G.; Dyall-Smith, M.L.
    Dihydrolipoamide dehydrogenase from the halophilic archaeon Haloferax volcanii: homologous overexpression of the cloned gene (1996), J. Bacteriol., 178, 3044-3048.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.8.1.4

Cloned(Commentary)

Cloned (Comment) Organism
overexpressed in the parent organism by using the halophilic archaeal rRNA promoter Haloferax volcanii

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.018
-
 dihydrolipoamide pH 8.0, 37°C Haloferax volcanii
0.08
-
 NAD+ pH 8.0, 37°C Haloferax volcanii

Metals/Ions

Metals/Ions Comment Organism Structure
KCl maximal enzymatic activity at 2 to 3 M KCl Haloferax volcanii

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
66500
-
 x * 66500, SDS-PAGE Haloferax volcanii

Organism

Organism UniProt Comment Textmining
Haloferax volcanii Q04829
-
-
Haloferax volcanii DSM 3757 Q04829
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Haloferax volcanii

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
24
-
 pH 8.0, 37°C Haloferax volcanii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
dihydrolipoamide + NAD+
-
 Haloferax volcanii lipoamide + NADH
-
 ?
dihydrolipoamide + NAD+
-
 Haloferax volcanii DSM 3757 lipoamide + NADH
-
 ?

Subunits

Subunits Comment Organism
? x * 66500, SDS-PAGE Haloferax volcanii

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9
-
-
 Haloferax volcanii