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Literature summary for 1.8.1.4 extracted from
- Characterization of interactions of dihydrolipoamide dehydrogenase with its binding protein in the human pyruvate dehydrogenase complex (2010), Biochem. Biophys. Res. Commun., 395, 416-419.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli BL21 cells | Homo sapiens |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| D413A | the mutant shows 85% activity in the forward reaction and 79% activity in the reverse reaction compared to the wild type enzyme | Homo sapiens |
| D413N | the mutant shows 119% activity in the forward reaction and 96% activity in the reverse reaction compared to the wild type enzyme | Homo sapiens |
| H348A | the mutant shows 60% activity in the forward reaction and 66% activity in the reverse reaction compared to the wild type enzyme | Homo sapiens |
| H348L | the mutant shows 65% activity in the forward reaction and 74% activity in the reverse reaction compared to the wild type enzyme | Homo sapiens |
| R447A | the mutant shows 110% activity in the forward reaction and 122% activity in the reverse reaction compared to the wild type enzyme | Homo sapiens |
| Y438A | the mutant shows 100% activity in the forward reaction and 91% activity in the reverse reaction compared to the wild type enzyme | Homo sapiens |
| Y438F | the mutant shows 100% activity in the forward reaction and 112% activity in the reverse reaction compared to the wild type enzyme | Homo sapiens |
| Y438H | the mutant shows 99% activity in the forward reaction and 92% activity in the reverse reaction compared to the wild type enzyme | Homo sapiens |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| Ni-NTA agarose column chromatography, gel filtration | Homo sapiens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| dihydrolipoamide + NAD+ | - |
Homo sapiens | lipoamide + NADH | - |
r |  |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| E3 | pyruvate dehydrogenase complex is composed of multiple copies of three catalytic components: pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2), and dihydrolipoamide dehydrogenase (E3) | Homo sapiens |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NAD+ | - |
Homo sapiens | |
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Release 2023.1 (January 2023)
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