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Literature summary for 1.8.1.4 extracted from
- Two proteins with diaphorase activity from Clostridium thermocellum and Moorella thermoacetica (2008), Biosci. Biotechnol. Biochem., 72, 877-879.
Application
| Application | Comment | Organism |
|---|---|---|
| degradation | shows flavin reductase activity with moderate diaphorase activity | Acetivibrio thermocellus |
| degradation | shows strong diaphorase activity | Moorella thermoacetica ATCC 39073 |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| ligated into vector pET-28a(+) and expressed in Escherichia coli BL21(DE3) as an N-terminal 6 x His-tag | Acetivibrio thermocellus |
| ligated into vector pET-28a(+) and expressed in Escherichia coli BL21(DE3) as an N-terminal 6 x His-tag | Moorella thermoacetica ATCC 39073 |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.015 | - |
2,6-dichlorophenolindophenol | - |
Acetivibrio thermocellus |  |
| 0.03 | - |
2,6-dichlorophenolindophenol | - |
Moorella thermoacetica ATCC 39073 | |
| 0.32 | - |
NADH | - |
Acetivibrio thermocellus | |
| 0.5 | - |
NADH | - |
Moorella thermoacetica ATCC 39073 | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 28206 | - |
x * 28206, sequence analysis | Acetivibrio thermocellus |
| 28430 | - |
x * 28430, sequence analysis | Moorella thermoacetica ATCC 39073 |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Acetivibrio thermocellus | - |
ATCC 27405T | - |
| Moorella thermoacetica ATCC 39073 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| by gel filtration | Acetivibrio thermocellus |
| by gel filtration | Moorella thermoacetica ATCC 39073 |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2,6-dichlorophenolindophenol + NADH + H+ | - |
Acetivibrio thermocellus | reduced 2,6-dichlorophenolindophenol + NAD+ | - |
r | |
| 2,6-dichlorophenolindophenol + NADH + H+ | - |
Moorella thermoacetica ATCC 39073 | reduced 2,6-dichlorophenolindophenol + NAD+ | - |
r | |
| oxidized 2,6-dichlorophenolindophenol + NADH | - |
Acetivibrio thermocellus | ? + NAD+ | - |
? | |
| oxidized 2,6-dichlorophenolindophenol + NADH | - |
Moorella thermoacetica ATCC 39073 | ? + NAD+ | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 28206, sequence analysis | Acetivibrio thermocellus |
| ? | x * 28430, sequence analysis | Moorella thermoacetica ATCC 39073 |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| diaphorase | - |
Acetivibrio thermocellus |
| diaphorase | - |
Moorella thermoacetica ATCC 39073 |
| NADH dehydrogenase | - |
Acetivibrio thermocellus |
| NADH dehydrogenase | - |
Moorella thermoacetica ATCC 39073 |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
- |
Acetivibrio thermocellus |
| 50 | - |
- |
Moorella thermoacetica ATCC 39073 |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| FMN | tightly bound | Moorella thermoacetica ATCC 39073 | |
| additional information | contains neither FMN nor FAD in the molecule | Acetivibrio thermocellus | |
| NADH | - |
Acetivibrio thermocellus | |
| NADH | - |
Moorella thermoacetica ATCC 39073 | |
| NADPH | oxidizes NADPH as efficiently as NADH | Acetivibrio thermocellus | |
| NADPH | oxidizes NADPH as efficiently as NADH | Moorella thermoacetica ATCC 39073 | |
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