Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.8.1.4 extracted from

  • Gazaryan, I.G.; Krasnikov, B.F.; Ashby, G.A.; Thorneley, R.N.; Kristal, B.S.; Brown, A.M.
    Zinc is a potent inhibitor of thiol oxidoreductase activity and stimulates reactive oxygen species production by lipoamide dehydrogenase (2002), J. Biol. Chem., 277, 10064-10072.
    View publication on PubMed
Search for more literature for 1.8.1.4

Activating Compound

Activating Compound Comment Organism Structure
Zn2+ stimulates the oxygen oxidase activity of the enzyme Sus scrofa

Inhibitors

Inhibitors Comment Organism Structure
NAD+ product inhibition of the oxygen oxidase activity Sus scrofa
Zn2+ reversible binding, competitive to lipoamide, uncompetitive to NADH Sus scrofa

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information dehydrogenase and oxidase activity kinetics Sus scrofa

Organism

Organism UniProt Comment Textmining
Sus scrofa
-
-
-

Purification (Commentary)

Purification (Comment) Organism
further purification of the commercial preparation by gel filtration Sus scrofa

Reaction

Reaction Comment Organism Reaction ID
protein N6-(dihydrolipoyl)lysine + NAD+ = protein N6-(lipoyl)lysine + NADH + H+ ping pong mechanism Sus scrofa
a

Source Tissue

Source Tissue Comment Organism Textmining
commercial preparation
-
 Sus scrofa
-
heart
-
 Sus scrofa
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
DL-6,8-thiooctic acid amide + NADH
-
 Sus scrofa ? + NAD+
-
 r
lipoamide + NADH
-
 Sus scrofa dihydrolipoamide + NAD+
-
 r
O2 + NADH the enzyme produces reactive oxygen species, i.e. hydrogen peroxide, acting as an oxidase Sus scrofa H2O2 + NAD+
-
 ?

Synonyms

Synonyms Comment Organism
LADH
-
 Sus scrofa
lipoamide dehydrogenase
-
 Sus scrofa

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
20
-
 assay at Sus scrofa

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 assay at Sus scrofa

Cofactor

Cofactor Comment Organism Structure
FAD
-
 Sus scrofa
NAD+
-
 Sus scrofa
NADH
-
 Sus scrofa

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
additional information
-
 additional information Zn2+ inhibition kinetics and mechanism Sus scrofa