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Literature summary for 1.8.1.4 extracted from
- Lipoyl dehydrogenase catalyzes reduction of nitrated DNA and protein adducts using dihydrolipoic acid or ubiquinol as the cofactor (2002), Chem. Biol. Interact., 140, 199-213.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| lipoic acid | increase in activity might be due to formation of dihydrolipoic acid in the assay system | Clostridium kluyveri |  |
| ubiquinone | slight increase in activity might be due to formation of ubiquinol in the assay system | Clostridium kluyveri | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| nitrated DNA + NAD(P)H | Sus scrofa | enzyme reduces DNA nitro adducts including 8-nitroguanine, 3-nitrotyrosine, and 8-nitroxanthine, which formed in presence of peroxynitrite and nitryl chloride present in inflamed tissues, the nitrated DNA adducts are unstable and undergo spontaneous depurination which can cause cancer, enzyme might be resonsible for reversing biological nitration processes | ? | - |
? | |
| nitrated DNA + NADPH | Clostridium kluyveri | enzyme reduces DNA nitro adducts including 8-nitroguanine, 3-nitrotyrosine, and 8-nitroxanthine, which formed in presence of peroxynitrite and nitryl chloride present in inflamed tissues, the nitrated DNA adducts are unstable and undergo spontaneous depurination | DNA + NADP+ + H2O | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Clostridium kluyveri | - |
- |
- |
| Sus scrofa | - |
- |
- |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| commercial preparation | - |
Sus scrofa | - |
| commercial preparation | - |
Clostridium kluyveri | - |
| heart | - |
Sus scrofa | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 3-nitrotyrosine + dihydrolipoic acid | - |
Sus scrofa | 3-aminotyrosine + lipoic acid + H2O | - |
? | |
| 3-nitrotyrosine + dihydrolipoic acid | - |
Clostridium kluyveri | 3-aminotyrosine + lipoic acid + H2O | - |
? | |
| 3-nitrotyrosine + NAD(P)H | - |
Sus scrofa | 3-aminotyrosine + NAD(P)+ + H2O | - |
? | |
| 3-nitrotyrosine + NADPH | - |
Clostridium kluyveri | 3-aminotyrosine + NADP+ + H2O | - |
? | |
| 3-nitrotyrosine + ubiquinol | - |
Sus scrofa | 3-aminotyrosine + ubiquinone + H2O | - |
? | |
| 3-nitrotyrosine + ubiquinol | - |
Clostridium kluyveri | 3-aminotyrosine + ubiquinone + H2O | - |
? | |
| 8-nitroguanine + dihydrolipoic acid | - |
Sus scrofa | 8-aminoguanine + lipoic acid + H2O | - |
? | |
| 8-nitroguanine + dihydrolipoic acid | - |
Clostridium kluyveri | 8-aminoguanine + lipoic acid + H2O | - |
? | |
| 8-nitroguanine + NAD(P)H | - |
Sus scrofa | 8-aminoguanine + NAD(P)+ + H2O | - |
? | |
| 8-nitroguanine + NADPH | - |
Clostridium kluyveri | 8-aminoguanine + NADP+ + H2O | - |
? | |
| 8-nitroguanine + ubiquinol | - |
Sus scrofa | 8-aminoguanine + ubiquinone + H2O | - |
? | |
| 8-nitroguanine + ubiquinol | - |
Clostridium kluyveri | 8-aminoguanine + ubiquinone + H2O | - |
? | |
| 8-nitroxanthine + dihydrolipoic acid | - |
Sus scrofa | 8-aminoxanthine + lipoic acid + H2O | - |
? | |
| 8-nitroxanthine + dihydrolipoic acid | - |
Clostridium kluyveri | 8-aminoxanthine + lipoic acid + H2O | - |
? | |
| 8-nitroxanthine + NAD(P)H | - |
Sus scrofa | 8-aminoxanthine + NAD(P)+ + H2O | - |
? | |
| 8-nitroxanthine + NADPH | - |
Clostridium kluyveri | 8-aminoxanthine + NADP+ + H2O | - |
? | |
| 8-nitroxanthine + ubiquinol | - |
Sus scrofa | 8-aminoxanthine + ubiquinone + H2O | - |
? | |
| 8-nitroxanthine + ubiquinol | - |
Clostridium kluyveri | 8-aminoxanthine + ubiquinone + H2O | - |
? | |
| additional information | substrate specificity, overview | Sus scrofa | ? | - |
? | |
| additional information | substrate specificity, overview | Clostridium kluyveri | ? | - |
? | |
| nitrated DNA + NAD(P)H | enzyme reduces DNA nitro adducts including 8-nitroguanine, 3-nitrotyrosine, and 8-nitroxanthine, which formed in presence of peroxynitrite and nitryl chloride present in inflamed tissues, the nitrated DNA adducts are unstable and undergo spontaneous depurination which can cause cancer, enzyme might be resonsible for reversing biological nitration processes | Sus scrofa | ? | - |
? | |
| nitrated DNA + NADPH | enzyme reduces DNA nitro adducts including 8-nitroguanine, 3-nitrotyrosine, and 8-nitroxanthine, which formed in presence of peroxynitrite and nitryl chloride present in inflamed tissues, the nitrated DNA adducts are unstable and undergo spontaneous depurination | Clostridium kluyveri | DNA + NADP+ + H2O | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| diaphorase | - |
Sus scrofa |
| diaphorase | - |
Clostridium kluyveri |
| lipoamide dehydrogenase | - |
Sus scrofa |
| lipoamide dehydrogenase | - |
Clostridium kluyveri |
| lipoyl dehydrogenase | - |
Sus scrofa |
| lipoyl dehydrogenase | - |
Clostridium kluyveri |
| NAD(P)H:lipoamide oxidoreductase | - |
Sus scrofa |
| NAD(P)H:lipoamide oxidoreductase | - |
Clostridium kluyveri |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Sus scrofa |
| 37 | - |
assay at | Clostridium kluyveri |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
assay at | Sus scrofa |
| 7 | - |
assay at | Clostridium kluyveri |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| dihydrolipoic acid | - |
Sus scrofa | |
| dihydrolipoic acid | - |
Clostridium kluyveri | |
| additional information | no activity with NADH | Clostridium kluyveri | |
| NAD(P)H | NADH is the preferred cofactor | Sus scrofa | |
| NADPH | - |
Clostridium kluyveri | |
| ubiquinol | - |
Sus scrofa | |
| ubiquinol | - |
Clostridium kluyveri | |
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