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Literature summary for 1.8.1.4 extracted from
- Lipoamide dehydrogenase from Malbranchea pulchella: isolation and characterization (1979), Biochemistry, 18, 4702-4707.
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 51000 | - |
2 * 51000, SDS-PAGE | Malbranchea pulchella |
| 102000 | - |
gel filtration | Malbranchea pulchella |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Malbranchea pulchella | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Malbranchea pulchella |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| mycelium | - |
Malbranchea pulchella | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Malbranchea pulchella |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| dihydrolipoamide + NAD+ | - |
Malbranchea pulchella | lipoamide + NADH | - |
? |  |
| additional information | diaphorase activity | Malbranchea pulchella | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 51000, SDS-PAGE | Malbranchea pulchella |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 65 | - |
170 min, 50% loss of activity | Malbranchea pulchella |
| 70 | - |
50 min, 50% loss of activity | Malbranchea pulchella |
| 75 | - |
50% loss of activity after 15 min, 50% loss of activity after 20 min in presence of NAD+, 50% loss of activity after less than 1 min in presence of dihydrolipoamide | Malbranchea pulchella |
| 80 | - |
6 min, 50% loss of activity | Malbranchea pulchella |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.2 | - |
- |
Malbranchea pulchella |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 10 | pH 5.0: about 10% of maximal activity, pH 7.0-7.5: 60-70% of maximal activity, pH 8.0-8.5: 90% of maximal activity, pH 10.0: 10% of maximal activity in carbonate buffer | Malbranchea pulchella |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| FAD | contains one noncovalently bound FAD per subunit | Malbranchea pulchella | |
| NAD+ | - |
Malbranchea pulchella | |
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