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Literature summary for 1.8.1.4 extracted from
- Lipoamide dehydrogenase from bakers yeast. Improved purification and some molecular, kinetic, and immunochemical properties (1983), Hoppe-Seyler's Z. Physiol. Chem., 364, 41-50.
General Stability
| General Stability | Organism |
|---|---|
| trypsin or papain, treatment with 0.019 mg/ml protease | Saccharomyces cerevisiae |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| NADH | competitive with respect to NAD+ | Saccharomyces cerevisiae |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.15 | - |
NAD+ | - |
Saccharomyces cerevisiae | |
| 0.48 | - |
dihydrolipoamide | - |
Saccharomyces cerevisiae | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 56000 | - |
2 * 56000, SDS-PAGE | Saccharomyces cerevisiae |
| 117000 | - |
gel filtration | Saccharomyces cerevisiae |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Saccharomyces cerevisiae | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Saccharomyces cerevisiae |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 843 | - |
- |
Saccharomyces cerevisiae |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| dihydrolipoamide + NAD+ | - |
Saccharomyces cerevisiae | lipoamide + NADH | - |
? | |
| hexacyanoferrate + NADH | - |
Saccharomyces cerevisiae | ? + NAD+ | - |
? | |
| iodonitrotetrazolium + NADH | - |
Saccharomyces cerevisiae | ? + NAD+ | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 56000, SDS-PAGE | Saccharomyces cerevisiae |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.5 | - |
- |
Saccharomyces cerevisiae |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| FAD | contains 1 FAD per subunit | Saccharomyces cerevisiae | |
| NAD+ | - |
Saccharomyces cerevisiae | |
| NADH | - |
Saccharomyces cerevisiae | |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.031 | - |
NADH | - |
Saccharomyces cerevisiae | |
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Release 2023.1 (January 2023)
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