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Literature summary for 1.8.1.20 extracted from

  • Khairy, H.; Wubbeler, J.; Steinbuchel, A.
    Biodegradation of the organic disulfide 4,4-dithiodibutyric acid by Rhodococcus spp (2015), Appl. Environ. Microbiol., 81, 8294-8306 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.8.1.20

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3)pLysS cells Rhodococcus erythropolis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4,4'-disulfanediyldibutanoate + NADH + H+ Rhodococcus erythropolis
-
 4-mercaptobutyric acid + NAD+
-
 ?
4,4'-disulfanediyldibutanoate + NADH + H+ Rhodococcus erythropolis MI2
-
 4-mercaptobutyric acid + NAD+
-
 ?
additional information Rhodococcus erythropolis no activity with NADPH ?
-
 ?
additional information Rhodococcus erythropolis MI2 no activity with NADPH ?
-
 ?

Organism

Organism UniProt Comment Textmining
Rhodococcus erythropolis A0A0N9HPS7
-
-
Rhodococcus erythropolis MI2 A0A0N9HPS7
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA column chromatography, and Superdex 200 gel filtration Rhodococcus erythropolis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4,4'-disulfanediyldibutanoate + NADH + H+
-
 Rhodococcus erythropolis 4-mercaptobutyric acid + NAD+
-
 ?
4,4'-disulfanediyldibutanoate + NADH + H+
-
 Rhodococcus erythropolis MI2 4-mercaptobutyric acid + NAD+
-
 ?
additional information no activity with NADPH Rhodococcus erythropolis ?
-
 ?
additional information no activity with NADPH Rhodococcus erythropolis MI2 ?
-
 ?

Subunits

Subunits Comment Organism
? x * 44000, SDS-PAGE Rhodococcus erythropolis

Synonyms

Synonyms Comment Organism
NOX
-
 Rhodococcus erythropolis

Cofactor

Cofactor Comment Organism Structure
NADH
-
 Rhodococcus erythropolis