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Literature summary for 1.8.1.2 extracted from

  • Zeng, J.; Wang, M.; Zhang, X.; Wang, Y.; Ai, C.; Liu, J.; Qiu, G.
    Expression, purification and characterization of the sulfite reductase hemo-subunit, SiR-HP, from Acidithiobacillus ferrooxidans (2008), Biotechnol. Lett., 30, 1239-1244.
    View publication on PubMed
Search for more literature for 1.8.1.2

Cloned(Commentary)

Cloned (Comment) Organism
the sulfite reductase hemo-subunit is cloned into the vector pLM1 for expression in Escherichia coli BL21DE3 cells Acidithiobacillus ferrooxidans

Protein Variants

Protein Variants Comment Organism
C427A mutation of the highly conserved cysteine 427, crucial residue for iron-sulfur cluster binding Acidithiobacillus ferrooxidans
C433A mutation of the highly conserved cysteine 427, crucial residue for iron-sulfur cluster binding Acidithiobacillus ferrooxidans
C472A mutation of the highly conserved cysteine 427, crucial residue for iron-sulfur cluster binding Acidithiobacillus ferrooxidans
C476A mutation of the highly conserved cysteine 427, crucial residue for iron-sulfur cluster binding Acidithiobacillus ferrooxidans

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
60000
-
 determined by SDS-PAGE Acidithiobacillus ferrooxidans

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
hydrogen sulfide + 3 NADP+ + 3 H2O Acidithiobacillus ferrooxidans
-
 sulfite + 3 NADPH + 3 H+
-
 ?

Organism

Organism UniProt Comment Textmining
Acidithiobacillus ferrooxidans
-
-
-

Purification (Commentary)

Purification (Comment) Organism
using a His-Trap column Acidithiobacillus ferrooxidans

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
hydrogen sulfide + 3 NADP+ + 3 H2O
-
 Acidithiobacillus ferrooxidans sulfite + 3 NADPH + 3 H+
-
 ?

Subunits

Subunits Comment Organism
? x * 60000, determined by SDS-PAGE Acidithiobacillus ferrooxidans

Synonyms

Synonyms Comment Organism
SIR
-
 Acidithiobacillus ferrooxidans
SIR-HP
-
 Acidithiobacillus ferrooxidans
sulfite reductase
-
 Acidithiobacillus ferrooxidans
sulfite reductase hemo-subunit
-
 Acidithiobacillus ferrooxidans

Cofactor

Cofactor Comment Organism Structure
FAD
-
 Acidithiobacillus ferrooxidans
FMN
-
 Acidithiobacillus ferrooxidans
iron-sulfur centre
-
 Acidithiobacillus ferrooxidans
NADPH
-
 Acidithiobacillus ferrooxidans
siroheme
-
 Acidithiobacillus ferrooxidans