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Literature summary for 1.8.1.2 extracted from
- A new type of sulfite reductase, a novel coenzyme F420-dependent enzyme, from the methanarchaeon Methanocaldococcus jannaschii (2005), J. Biol. Chem., 280, 38776-38786.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| coenzyme F420 | - |
Methanocaldococcus jannaschii |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0122 | - |
sulfite | within a sulfite concentration range of 0.0014-0.3 mM and with a fixed reduced F420 concentration of 0.04 mM | Methanocaldococcus jannaschii | |
| 0.021 | - |
reduced F420 | at a fixed sulfite concentration of 0.29 mM and within a reduced F420 concentration range of 0.004-0.06 mM | Methanocaldococcus jannaschii | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 70000 | - |
SDS-PAGE | Methanocaldococcus jannaschii |
| 350000 | - |
gel filtration | Methanocaldococcus jannaschii |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Methanocaldococcus jannaschii | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| phenyl-Sepharose chromatography, F420-Sepharose chromatography and QAE-Sephadex gel filtration | Methanocaldococcus jannaschii |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.0013 | - |
in 50 mM potassium phosphate buffer (pH 7), 0.044 mM reduced F420, and 1.5 mM sodium sulfite, from cell extract | Methanocaldococcus jannaschii |
| 0.0182 | - |
in 50 mM potassium phosphate buffer (pH 7), 0.044 mM reduced F420, and 1.5 mM sodium sulfite, after 14fold purification | Methanocaldococcus jannaschii |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| sulfite + reduced F420 | - |
Methanocaldococcus jannaschii | sulfide + oxidized F420 | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| coenzyme F420-dependent sulfite reductase | - |
Methanocaldococcus jannaschii |
| Fsr | - |
Methanocaldococcus jannaschii |
| sulfite reductase | - |
Methanocaldococcus jannaschii |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 95 | - |
- |
Methanocaldococcus jannaschii |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
- |
Methanocaldococcus jannaschii |
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