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Literature summary for 1.8.1.2 extracted from
- Studies on yeast sulfite reductase. I. Purification and characterization (1968), Biochim. Biophys. Acta, 153, 555-575.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 2'-AMP | NADPH-sulfite reductase and NADPH-cytochrome c reductase | Saccharomyces cerevisiae |  |
| arsenite | - |
Saccharomyces cerevisiae | |
| CN- | only when added with NADPH prior to substrate, sulfite prevents NADPH-reduced form from irreversible conversion by CN-; severe inhibition of NADPH-sulfite,-nitrite,-hydroxylamine reductase, no inhibition of methyl viologen-sulfite reductase, small extent of inhibition of NADPH dependent reduction of cytochrome c, ferricyanide, quinones etc. | Saccharomyces cerevisiae | |
| NADP+ | NADPH-dependent activities | Saccharomyces cerevisiae | |
| p-chloromercuribenzoate | NADPH-dependent activities | Saccharomyces cerevisiae | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.012 | 0.017 | sulfite | - |
Saccharomyces cerevisiae | |
| 0.8 | 1.5 | nitrite | - |
Saccharomyces cerevisiae | |
| 4.5 | 10.5 | hydroxylamine | - |
Saccharomyces cerevisiae | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| soluble | - |
Saccharomyces cerevisiae | - |
- |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 350000 | - |
calculation from FAD and FMN content, sedimentation coefficient | Saccharomyces cerevisiae |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Saccharomyces cerevisiae | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Saccharomyces cerevisiae |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 1.85 | - |
- |
Saccharomyces cerevisiae |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, 0.3 M phosphate buffer pH 7.3, 1 year stable stable | Saccharomyces cerevisiae |
| 4°C, 0.3 M phosphate buffer pH 7.3, 1 week stable | Saccharomyces cerevisiae |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| hydroxylamine + NADPH | - |
Saccharomyces cerevisiae | ammonia + NADP+ | - |
? | |
| additional information | reactions catalyzed by flavoprotein subunit alone: transfer of electrons from NADPH to cytochrome c, ferricyanide, dichlorphenolindophenol, menadione, FMN, FAD, O2 | Saccharomyces cerevisiae | ? | - |
? | |
| nitrite + NADPH | - |
Saccharomyces cerevisiae | ammonia + NADP+ | - |
? | |
| sulfite + NADPH | NADPH can be replaced by reduced methyl viologen | Saccharomyces cerevisiae | sulfide + NADP+ + H2O | - |
? | |
| sulfite + NADPH | benzyl viologen | Saccharomyces cerevisiae | sulfide + NADP+ + H2O | - |
? | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | 7.5 | NADPH-sulfite reductase | Saccharomyces cerevisiae |
| 7.5 | 7.9 | NADPH-cytochrome c reductase | Saccharomyces cerevisiae |
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