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Literature summary for 1.8.1.2 extracted from

  • Yoshimoto, A.; Naiki, N.; Sato, R.
    Sulfite reductase (bakers¦ yeast) (1971), Methods Enzymol., 17B, 520-528.
No PubMed abstract available
Search for more literature for 1.8.1.2

General Stability

General Stability Organism
low ionic strength such as 0.01 M phosphate buffer destroys NADPH-dependent activities Saccharomyces cerevisiae

Inhibitors

Inhibitors Comment Organism Structure
arsenite
-
 Saccharomyces cerevisiae
CN- severe inhibition of NADPH-sulfite,-nitrite,-hydroxylamine reductase, no inhibition of methyl viologen-sulfite reductase, small extent of inhibition of NADPH dependent reduction of cytochrome c, ferricyanide, quinones etc. Saccharomyces cerevisiae
NADP+ NADPH-dependent activities Saccharomyces cerevisiae
p-chloromercuribenzoate NADPH-dependent activities Saccharomyces cerevisiae
Sulfide reduction of sulfite, nitrite, hydroxylamine by reduced methyl viologen Saccharomyces cerevisiae

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.012 0.017 sulfite
-
 Saccharomyces cerevisiae
0.018
-
 NADPH reduction of cytochrome c Saccharomyces cerevisiae
0.021
-
 NADPH reduction of sulfite Saccharomyces cerevisiae
0.06
-
 cytochrome c
-
 Saccharomyces cerevisiae
0.8 1.5 nitrite
-
 Saccharomyces cerevisiae
4.5 10.5 hydroxylamine
-
 Saccharomyces cerevisiae

Localization

Localization Comment Organism GeneOntology No. Textmining
soluble
-
 Saccharomyces cerevisiae
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
350000
-
 calculation from FAD and FMN content, sedimentation coefficient Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
sulfite + NADPH + H+ Saccharomyces cerevisiae
-
 sulfide + NADP+ + H2O
-
 ?

Organic Solvent Stability

Organic Solvent Comment Organism
Acetone inactivation Saccharomyces cerevisiae
Ethanol inactivation Saccharomyces cerevisiae

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Saccharomyces cerevisiae

Storage Stability

Storage Stability Organism
-20°C, 0.3 M phosphate buffer pH 7.3, 1 year stable stable Saccharomyces cerevisiae
4°C, 0.3 M phosphate buffer pH 7.3, 1 week stable Saccharomyces cerevisiae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
hydroxylamine + NADPH
-
 Saccharomyces cerevisiae ammonia + NADP+
-
 ?
additional information overview on assay methods Saccharomyces cerevisiae ?
-
 ?
additional information reactions catalyzed by flavoprotein subunit alone: transfer of electrons from NADPH to cytochrome c, ferricyanide, dichlorphenolindophenol, menadione, FMN, FAD, O2 Saccharomyces cerevisiae ?
-
 ?
NADP+ + ferrocytochrome c
-
 Saccharomyces cerevisiae NADPH + H+ + ferricytochrome c
-
 r
nitrite + NADPH
-
 Saccharomyces cerevisiae ammonia + NADP+
-
 ?
sulfite + NADPH
-
 Saccharomyces cerevisiae sulfide + NADP+ + H2O
-
 ?
sulfite + NADPH + H+
-
 Saccharomyces cerevisiae sulfide + NADP+ + H2O
-
 ?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7 7.5 NADPH-sulfite reductase Saccharomyces cerevisiae
7.5 7.9 NADPH-cytochrome c reductase Saccharomyces cerevisiae
7.7 8.5 methyl viologen-sulfite reductase Saccharomyces cerevisiae

Cofactor

Cofactor Comment Organism Structure
flavin treatment with ammonium sulfate causes dissociation of FMN but retetion of FAD and heme Saccharomyces cerevisiae