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Literature summary for 1.8.1.12 extracted from
- Site-directed mutagenesis of the redox-active cysteines of Trypanosoma cruzi trypanothione reductase (1995), Eur. J. Biochem., 228, 745-752.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| additional information | activity is dependent on a redox-active cysteine at the active centre | Trypanosoma cruzi |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| overexpression of wild-type and mutants C53S, C53A, C58S in Escherichia coli | Trypanosoma cruzi |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| C53A | site-directed mutagenesis, without catalytic active cysteine residue in the active center, no activity with trypanothione disulfide and NADPH, but showing transhydrogenase activity between NADPH and thio-NADP+ | Trypanosoma cruzi |
| C53S | site-directed mutagenesis, without catalytic active cysteine residue in the active center, no activity with trypanothione disulfide and NADPH, but showing transhydrogenase activity between NADPH and thio-NADP+ | Trypanosoma cruzi |
| C58S | site-directed mutagenesis, without catalytic active cysteine residue in the active center, no activity with trypanothione disulfide and NADPH, but showing transhydrogenase activity between NADPH and thio-NADP+ | Trypanosoma cruzi |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.004 | 0.015 | thio-NADP+ | recombinant mutants C53S, C53A, C58S | Trypanosoma cruzi |  |
| 0.007 | 0.014 | NADPH | recombinant mutants C53S, C53A, C58S | Trypanosoma cruzi | |
| 0.017 | - |
NADPH | recombinant wild-type | Trypanosoma cruzi | |
| 0.024 | - |
trypanothione disulfide | recombinant wild-type | Trypanosoma cruzi | |
| 0.046 | - |
glutathionylspermidine disulfide | recombinant wild-type | Trypanosoma cruzi | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Trypanosoma cruzi | - |
Silvio strain | - |
| Trypanosoma cruzi Silvio | - |
Silvio strain | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant wild-type and mutants C53S, C53A, C58S from Escherichia coli | Trypanosoma cruzi |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.18 | - |
recombinant mutant C58S: transhydrogenase activity between NADPH and thio-NADP+ | Trypanosoma cruzi |
| 0.21 | - |
recombinant mutant C53A: transhydrogenase activity between NADPH and thio-NADP+ | Trypanosoma cruzi |
| 0.44 | - |
recombinant mutant C53S: transhydrogenase activity between NADPH and thio-NADP+ | Trypanosoma cruzi |
| 6.49 | - |
recombinant wild-type: transhydrogenase activity between NADPH and thio-NADP+ | Trypanosoma cruzi |
| 143.2 | - |
purified recombinant enzyme | Trypanosoma cruzi |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| glutathionylspermidine disulfide + NADPH | - |
Trypanosoma cruzi | 2-glutathionylspermidine + NADP+ | - |
? | |
| glutathionylspermidine disulfide + NADPH | - |
Trypanosoma cruzi Silvio | 2-glutathionylspermidine + NADP+ | - |
? | |
| additional information | wild-type enzyme shows transhydrogenase activity between NADPH and thio-NADP+ | Trypanosoma cruzi | ? | - |
? | |
| additional information | recombinant mutants C53S, C53A, C58S: no activity with trypanothione disulfide and NADPH, but showing transhydrogenase activity between NADPH and thio-NADP+ | Trypanosoma cruzi | ? | - |
? | |
| additional information | wild-type enzyme shows transhydrogenase activity between NADPH and thio-NADP+ | Trypanosoma cruzi Silvio | ? | - |
? | |
| additional information | recombinant mutants C53S, C53A, C58S: no activity with trypanothione disulfide and NADPH, but showing transhydrogenase activity between NADPH and thio-NADP+ | Trypanosoma cruzi Silvio | ? | - |
? | |
| NADPH + H+ + thio-NADP+ | - |
Trypanosoma cruzi | NADP+ + thio-NADPH + H+ | - |
r | |
| NADPH + H+ + thio-NADP+ | - |
Trypanosoma cruzi Silvio | NADP+ + thio-NADPH + H+ | - |
r | |
| trypanothione disulfide + NADPH | - |
Trypanosoma cruzi | trypanothione + NADP+ | - |
? | |
| trypanothione disulfide + NADPH | - |
Trypanosoma cruzi Silvio | trypanothione + NADP+ | - |
? | |
| trypanothione disulfide + thio-NADPH + H+ | - |
Trypanosoma cruzi | trypanothione + thio-NADP+ | - |
r | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| TR | - |
Trypanosoma cruzi |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
assay at | Trypanosoma cruzi |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.19 | 0.47 | thio-NADPH | recombinant mutants C53S, C53A, C58S | Trypanosoma cruzi | |
| 0.21 | 0.51 | NADPH | recombinant mutants C53S, C53A, C58S | Trypanosoma cruzi | |
| 6.33 | - |
NADPH | recombinant wild-type | Trypanosoma cruzi | |
| 7.02 | - |
thio-NADPH | recombinant wild-type | Trypanosoma cruzi | |
| 85.4 | - |
glutathionylspermidine disulfide | recombinant wild-type | Trypanosoma cruzi | |
| 108 | - |
trypanothione disulfide | recombinant wild-type | Trypanosoma cruzi | |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| beta-thio-NADPH | - |
Trypanosoma cruzi | |
| FAD | - |
Trypanosoma cruzi | |
| NADPH | - |
Trypanosoma cruzi | |
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