Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.8.1.12 extracted from

  • Davioud-Charvet, E.; Becker, K.; Landry, V.; Gromer, S.; Loge, C.; Sergheraert, C.
    Synthesis of 5,5'-dithiobis(2-nitrobenzamides) as alternative substrates for trypanothione reductase and thioredoxin reductase: a microtiter colorimetric assay for inhibitor screening (1999), Anal. Biochem., 268, 1-8.
    View publication on PubMed
Search for more literature for 1.8.1.12

Activating Compound

Activating Compound Comment Organism Structure
additional information activity is dependent on a redox-active cysteine at the active centre Trypanosoma cruzi

Application

Application Comment Organism
medicine potential target for drug design against trypanosomiasis Trypanosoma cruzi

Cloned(Commentary)

Cloned (Comment) Organism
overexpression in Escherichia coli Trypanosoma cruzi

Inhibitors

Inhibitors Comment Organism Structure
5,5'-dithiobis(N-[3-(4-methylpiperazin-1-yl)propyl]-2-nitrobenzamide) recombinant enzyme Trypanosoma cruzi
clomipramine recombinant enzyme Trypanosoma cruzi

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.035
-
 5,5'-dithiobis(N-[3-(dimethylamino)propyl]-2-nitrobenzamide) recombinant enzyme Trypanosoma cruzi
0.045
-
 trypanothione disulfide recombinant enzyme Trypanosoma cruzi
0.3
-
 5,5'-dithiobis(N-[3-(4-methylpiperazin-1-yl)propyl]-2-nitrobenzamide) recombinant enzyme Trypanosoma cruzi

Organism

Organism UniProt Comment Textmining
Trypanosoma cruzi
-
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
137
-
 recombinant purified enzyme Trypanosoma cruzi

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5,5'-dithiobis(N-[3-(4-methylpiperazin-1-yl)propyl]-2-nitrobenzamide) + NADPH + H+
-
 Trypanosoma cruzi ?
-
 r
5,5'-dithiobis(N-[3-(dimethylamino)propyl]-2-nitrobenzamide) + NADPH + H+
-
 Trypanosoma cruzi ?
-
 ?
5,5'-dithiobis(N-[3-(dimethylamino)propyl]-2-nitrobenzamide) + NADPH + H+
-
 Trypanosoma cruzi ?
-
 r
additional information reduces 5,5'-dithiobis(2-nitrobenzamides) Trypanosoma cruzi ?
-
 ?
trypanothione disulfide + NADPH
-
 Trypanosoma cruzi trypanothione + NADP+
-
 ?

Synonyms

Synonyms Comment Organism
TR
-
 Trypanosoma cruzi

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
 assay at Trypanosoma cruzi

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
125
-
 5,5'-dithiobis(N-[3-(4-methylpiperazin-1-yl)propyl]-2-nitrobenzamide) recombinant enzyme Trypanosoma cruzi
125
-
 5,5'-dithiobis(N-[3-(dimethylamino)propyl]-2-nitrobenzamide) recombinant enzyme Trypanosoma cruzi
240
-
 trypanothione disulfide recombinant enzyme Trypanosoma cruzi

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.25
-
 assay at Trypanosoma cruzi

Cofactor

Cofactor Comment Organism Structure
FAD
-
 Trypanosoma cruzi
NADPH
-
 Trypanosoma cruzi

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.0086
-
 clomipramine recombinant enzyme, with 5,5'-dithiobis(N-[3-(dimethylamino)propyl]-2-nitrobenzamide) Trypanosoma cruzi