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Literature summary for 1.8.1.12 extracted from

  • Gallwitz, H.; Bonse, S.; Martinez-Cruz, A.; Schlichting, I.; Schumacher, K.; Krauth-Siegel, R.L.
    Ajoene is an inhibitor and subversive substrate of human glutathione reductase and Trypanosoma cruzi trypanothione reductase: crystallographic, kinetic, and spectroscopic studies (1999), J. Med. Chem., 42, 364-372.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
additional information activity is dependent on a redox-active cysteine at the active centre Trypanosoma cruzi

Inhibitors

Inhibitors Comment Organism Structure
ajoene i.e. (E,Z)-4,5,9-trithiadodeca-1,6,11-triene-9-oxide, natural compound from garlic, Allium sativum, covalent inhibition, but also substrate; time- and temperature-dependent inhibition Trypanosoma cruzi

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2
-
(E,Z)-ajoene above, with NADPH Trypanosoma cruzi

Organism

Organism UniProt Comment Textmining
Trypanosoma cruzi
-
-
-

Reaction

Reaction Comment Organism Reaction ID
trypanothione + NADP+ = trypanothione disulfide + NADPH + H+ mechanistic reaction scheme, structure-function relation Trypanosoma cruzi

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(E,Z)-ajoene + NADPH + H+
-
Trypanosoma cruzi ?
-
r
ajoene + NADPH i.e. (E,Z)-4,5,9-trithiadodeca-1,6,11-triene-9-oxide, substrate and inhibitor Trypanosoma cruzi ?
-
?
trypanothione disulfide + NADPH
-
Trypanosoma cruzi trypanothione + NADP+
-
?

Synonyms

Synonyms Comment Organism
TR
-
Trypanosoma cruzi

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.67
-
(E,Z)-ajoene
-
Trypanosoma cruzi

Cofactor

Cofactor Comment Organism Structure
FAD
-
Trypanosoma cruzi
NADPH
-
Trypanosoma cruzi