Literature summary for 1.8.1.10 extracted from

delCardayre, S.B.; Davies, J.E.
Staphylococcus aureus coenzyme A disulfide reductase, a new subfamily of pyridine nucleotide-disulfide oxidoreductase (1998), J. Biol. Chem., 273, 5752-5757.

Search for more literature for 1.8.1.10

Application

Application	Comment	Organism
medicine	potential point of intervention in the treatment of staphylococcal infections	Staphylococcus aureus
pharmacology	anti-staphylococcal agent, possible target for the design of selective inhibitors that would interrupt the thiol metabolism of the human pathogen Staphylococcus aureus	Staphylococcus aureus

Cloned(Commentary)

Cloned (Comment)	Organism
cdr gene encoding coenzyme A glutathione disulfide reductase is cloned, sequenced and overexpressed in Escherichia coli BL21 using the pET expression system, gene encodes a 438-amino acid polypeptide	Staphylococcus aureus

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.0019	-	NADPH	recombinant enzyme expressed in E. coli	Staphylococcus aureus
0.014	-	CoA disulfide	recombinant enzyme expressed in E. coli	Staphylococcus aureus

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
49200	-	2 * 49200, calculated from the nucleotide sequence	Staphylococcus aureus
90000	-	native molecular weight	Staphylococcus aureus

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
CoA disulfide + NADPH	Staphylococcus aureus	central role in thiol metabolism	CoA + NADP+	-	?
CoA disulfide + NADPH	Staphylococcus aureus NCTC 8325	central role in thiol metabolism	CoA + NADP+	-	?

Organism

Organism	UniProt	Comment	Textmining
Staphylococcus aureus	-	strain 8325-4	-
Staphylococcus aureus NCTC 8325	-	strain 8325-4	-

Purification (Commentary)

Purification (Comment)	Organism
purification of recombinant CoADR, expressed in Escherichia coli	Staphylococcus aureus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
additional information	-	-	Staphylococcus aureus

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
CoA disulfide + NADPH	substrate CoASSCoA, enzyme forms during catalysis a stable mixed disulfide intermediate with CoA	Staphylococcus aureus	CoA + NADP+	-	?
CoA disulfide + NADPH	central role in thiol metabolism	Staphylococcus aureus	CoA + NADP+	-	?
CoA disulfide + NADPH	substrate CoASSCoA, enzyme forms during catalysis a stable mixed disulfide intermediate with CoA	Staphylococcus aureus NCTC 8325	CoA + NADP+	-	?
CoA disulfide + NADPH	central role in thiol metabolism	Staphylococcus aureus NCTC 8325	CoA + NADP+	-	?

Subunits

Subunits	Comment	Organism
dimer	2 * 49200, calculated from the nucleotide sequence	Staphylococcus aureus

Synonyms

Synonyms	Comment	Organism
CoA disulfide reductase	-	Staphylococcus aureus
CoADR	-	Staphylococcus aureus
More	subfamily of pyridine nucleotide-disulfide oxidoreductase	Staphylococcus aureus

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
1100	-	CoA disulfide	-	Staphylococcus aureus

Cofactor

Cofactor	Comment	Organism	Structure
FAD	flavoprotein, gene with two FAD-binding regions	Staphylococcus aureus
NADPH	NADPH-dependent	Staphylococcus aureus
NADPH	gene with one NADPH-binding region	Staphylococcus aureus

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Release 2023.1 (January 2023)