General Stability | Organism |
---|---|
the enzyme is most stable in the absence of salt | Haloferax denitrificans |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
azide | competitive inhibitor with respect to nitrate | Haloferax denitrificans | |
Chlorate | competitive inhibitor with respect to nitrate | Haloferax denitrificans | |
cyanide | preincubating the enzyme with cyanide increases the extent of inhibition which in turn is intensified when dithionite is present. Although cyanide is a noncompetitive inhibitor with respect to nitrate, nitrate protects against inhibition | Haloferax denitrificans | |
thiocyanate | 10 mM, 53% inhibition. Preincubating for 1 h does not increase the inhibition | Haloferax denitrificans |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0028 | - |
reduced methyl viologen | 37°C, pH 7.2 | Haloferax denitrificans | |
0.19 | - |
nitrate | 37°C, pH 7.2 | Haloferax denitrificans |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | - |
Haloferax denitrificans | 16020 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
KCl | maximum activity occurrs in the absence of NaCl and decreases with increasing concentrations of NaCl so that the activity is 46% of maximum when the enzyme is assayed in the presence of 4 M NaCl. Replacing NaCl with KCl does not significantly change the response | Haloferax denitrificans | |
NaCl | maximum activity occurrs in the absence of NaCl and decreases with increasing concentrations of NaCl so that the activity is 46% of maximum when the enzyme is assayed in the presence of 4 M NaCl. Replacing NaCl with KCl does not significantly change the response | Haloferax denitrificans |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
60000 | - |
2 * 116000 + 2 * 60000, SDS-pAGE | Haloferax denitrificans |
116000 | - |
2 * 116000 + 2 * 60000, SDS-pAGE | Haloferax denitrificans |
380000 | - |
gel filtration | Haloferax denitrificans |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Haloferax denitrificans | - |
- |
- |
Haloferax denitrificans DSM 4425 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Haloferax denitrificans |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
62 | - |
37°C, pH 7.2 | Haloferax denitrificans |
Storage Stability | Organism |
---|---|
-19°C, 3 months, enzyme loses no activity | Haloferax denitrificans |
4°C, 8 days, in the absence of NaCl, little if any loss of activity | Haloferax denitrificans |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
chlorate + reduced acceptor | - |
Haloferax denitrificans | chlorite + acceptor | - |
? | |
chlorate + reduced acceptor | - |
Haloferax denitrificans DSM 4425 | chlorite + acceptor | - |
? | |
nitrate + reduced methyl viologen | - |
Haloferax denitrificans | nitrite + methyl viologen | - |
? | |
nitrate + reduced methyl viologen | - |
Haloferax denitrificans DSM 4425 | nitrite + methyl viologen | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | 2 * 116000 + 2 * 60000, SDS-pAGE | Haloferax denitrificans |
Synonyms | Comment | Organism |
---|---|---|
dissimilatory nitrate reductase | - |
Haloferax denitrificans |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Haloferax denitrificans |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.2 | - |
- |
Haloferax denitrificans |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6 | 8 | the activities at pH 6.0 and pH 8.0 are 83% and 81% of the activity at pH 7.2 | Haloferax denitrificans |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0007 | - |
azide | 37°C, pH 7.2 | Haloferax denitrificans | |
0.5 | - |
cyanide | 37°C, pH 7.2 | Haloferax denitrificans | |
0.8 | - |
Chlorate | 37°C, pH 7.2 | Haloferax denitrificans |